Maksiutov R A, Shchelkunov S N
Mol Gen Mikrobiol Virusol. 2011(2):30-4.
Efficacy of candidate DNA-vaccines based on the variola virus natural gene A30L and artificial gene A30Lopt with modified codon usage, optimized for expression in mammalian cells, was tested. The groups of mice were intracutaneously immunized three times with three-week intervals with candidate DNA-vaccines: pcDNA_A30L or pcDNA_A30Lopt, and in three weeks after the last immunization all mice in the groups were intraperitoneally infected by the ectromelia virus K1 strain in 10 LD50 dose for the estimation of protection. It was shown that the DNA-vaccines based on natural gene A30L and codon-optimized gene A30Lopt elicited virus, thereby neutralizing the antibody response and protected mice from lethal intraperitoneal challenge with the ectromelia virus with lack of statistically significant difference.
对基于天花病毒天然基因A30L和密码子使用经过修饰、针对在哺乳动物细胞中表达进行优化的人工基因A30Lopt的候选DNA疫苗的效力进行了测试。将小鼠分组，每隔三周用候选DNA疫苗pcDNA_A30L或pcDNA_A30Lopt进行三次皮内免疫，在最后一次免疫后三周，对各组所有小鼠腹腔注射10 LD50剂量的埃可病毒K1株，以评估保护效果。结果表明，基于天然基因A30L和密码子优化基因A30Lopt的DNA疫苗引发了病毒中和抗体反应，并保护小鼠免受埃可病毒致死性腹腔攻击，且无统计学显著差异。
