Iakovin N A, Fesenko I A, Isachkin A V, Karlov G I
Genetika. 2011 May;47(5):643-50.
Using five SSR markers, polymorphism ofmicrosatellite loci was examined in 46 cultivars and five species of pear (Pyrus ussuriensis, P. bretscgneideri, P. pyraster, and P. elaegnifolia). Most of the accessions examined demonstrated the presence of unique allele sets. The degree of relationship between Russian and Western European pear cultivar was established. It was demonstrated that P. ussuriensis and its first generation progeny were genetically distant from typical cultivars of P. communis, as well as from the P. communis x P. ussuriensis hybrids of later generations. SSR estimates of the cultivar relatedness were shown to correlate with the corresponding pedigree-based estimates. A number of SSR alleles specific to P. ussuriensis were identified. Based on the analysis of microsatellite loci, the allelic composition was determined for each cultivar examined. These data can serve as a molecular certificate of the cultivar.
利用5个SSR标记，对46个梨品种以及5个梨种（秋子梨、白梨、西洋梨和沙梨）的微卫星位点多态性进行了检测。大多数检测的种质都表现出独特的等位基因组合。确定了俄罗斯梨品种与西欧梨品种之间的亲缘关系。结果表明，秋子梨及其第一代后代在遗传上与西洋梨的典型品种以及西洋梨与秋子梨杂交的后代有较远的距离。品种亲缘关系的SSR估计值与相应的基于系谱的估计值相关。鉴定出了一些秋子梨特有的SSR等位基因。基于微卫星位点分析，确定了每个检测品种的等位基因组成。这些数据可作为品种的分子鉴定依据。
