Iliaskin A V, Baturina G S, Medvedev D A, Ershov A P, Solenov E I
Biofizika. 2011 May-Jun;56(3):550-60.
The regulatory decrease in the volume of principal cells of collecting ducts to hypoosmotic shock has been investigated experimentally and using the mathematical modeling. A mathematical model of the response of collecting duct principal cells to hypotonic shock has been constructed on the basis of the experimental time course of changes in cell volume measured by the fluorescent dye Calcein. It was shown that the regulatory decrease in volume under hypotonic conditions occurs via a marked release of osmolytes and is accompanied by a decrease in water permeability of the cell membrane. The mathematical modeling of transmembrane transport processes allowed us to quantitatively estimate the changes in membrane water permeability, which decreased tenfold, from 2 x 10(-1) cm/s to 2 x 10(-2) cm/s. It was also shown that the effective regulatory decrease in the volume of collecting duct principal cells in hypotonic medium results from a significant increase in membrane permeability for K+, Cl-, and organic anions.
已通过实验和数学建模对集合管主细胞体积对低渗休克的调节性减少进行了研究。基于用荧光染料钙黄绿素测量的细胞体积变化的实验时间进程，构建了集合管主细胞对低渗休克反应的数学模型。结果表明，低渗条件下的调节性体积减少是通过显著释放渗透溶质发生的，并伴随着细胞膜水渗透性的降低。跨膜运输过程的数学建模使我们能够定量估计膜水渗透性的变化，其降低了十倍，从2×10⁻¹厘米/秒降至2×10⁻²厘米/秒。还表明，低渗介质中集合管主细胞体积的有效调节性减少是由于K⁺、Cl⁻和有机阴离子的膜渗透性显著增加所致。
