Department of Physiology, Medical School, University of Thessaly, Biopolis, Larissa, Greece.
Math Biosci. 2013 Aug;244(2):176-87. doi: 10.1016/j.mbs.2013.05.007. Epub 2013 May 31.
Outer Medullary Collecting Duct (OMCD) principal cells are exposed to significant changes of the extracellular osmolarity and thus the analysis of their regulatory volume decrease (RVD) function is of great importance in order to avoid cell membrane rupture and subsequent death. In this paper we provide a sub-second temporal analysis of RVD events occurring after two successive hypo-osmotic challenges in rat kidney OMCD principal cells. We performed experimental cell volume measurements and created a mathematical model based on our experimental results. As a consequence of RVD the cell expels part of intracellular osmolytes and reduces the permeability of the plasma membrane to water. The next osmotic challenge does not cause significant RVD if it occurs within a minute after the primary shock. In such a case the cell reacts as an ideal osmometer. Through our model we provide the basis for further detailed studies on RVD dynamical modeling.
外髓集合管（OMCD）主细胞暴露于显著变化的细胞外渗透压，因此，分析其调节性容积减少（RVD）功能非常重要，以避免细胞膜破裂和随后的死亡。在本文中，我们提供了大鼠肾脏 OMCD 主细胞在两次连续低渗刺激后发生的 RVD 事件的亚秒级时间分析。我们进行了实验细胞体积测量，并根据我们的实验结果创建了一个数学模型。作为 RVD 的结果，细胞排出部分细胞内渗透压溶质，并降低质膜对水的通透性。如果下一次渗透压刺激在第一次冲击后一分钟内发生，则不会引起明显的 RVD。在这种情况下，细胞作为理想渗透压计反应。通过我们的模型，我们为进一步详细研究 RVD 动力学建模提供了基础。
