金雀异黄素抑制裸鼠溶骨性骨转移并增强骨矿物质。

Genistein inhibits osteolytic bone metastasis and enhances bone mineral in nude mice.

机构信息

Department of Environmental Epidemiology and Bone Toxicology, Institute of Radiation Medicine, Fudan University, No. 2094 Xietu Road, Shanghai 200032, China.

出版信息

Environ Toxicol Pharmacol. 2010 Jul;30(1):37-44. doi: 10.1016/j.etap.2010.03.016. Epub 2010 Mar 31.

DOI:10.1016/j.etap.2010.03.016

PMID:21787627

Abstract

In this study, the effective activity of genistein on osteolytic bone metastasis and bone mineral was investigated. Female BALB/c-nu/nu mice were injected with estrogen receptor-negative human breast cancer cells, MDA-MB-231, into left cardiac ventricle to form osteolytic bone metastases, and administered genistein subcutaneously after radiologically small but defined osteolytic metastases had been observed (protocol 1), simultaneously with cancer cells inoculation (protocol 2) and prophylactically 7 days before inoculation of cancer cells (protocol 3). In all protocols, genistein (10mg/kg/day) markedly reduced the number and volume of osteolytic bone metastases assessed by radiography and the number of osteoclasts. Furthermore, histomorphometrical analysis revealed that genistein markedly increased trabecular area (Tb.Ar%), trabecular thickness (Tb.Th) and trabecular number (Tb.N), and decreased trabecular separation (Tb.Sp). These results thus demonstrate that genistein could inhibit osteolytic bone metastases, suppress bone resorption, increase bone mass and improve bone microstructure in bone metastases of breast cancer.

摘要

在这项研究中，研究了染料木黄酮对溶骨性骨转移和骨矿物质的有效活性。将雌激素受体阴性的人乳腺癌细胞 MDA-MB-231 注入雌性 BALB/c-nu/nu 小鼠的左心室，形成溶骨性骨转移，然后在影像学上观察到小但明确的溶骨性转移后（方案 1）、同时接种癌细胞（方案 2）和在接种癌细胞前 7 天预防性地（方案 3）皮下给予染料木黄酮。在所有方案中，染料木黄酮（10mg/kg/天）显著减少了通过放射照相术评估的溶骨性骨转移的数量和体积以及破骨细胞的数量。此外，组织形态计量学分析显示，染料木黄酮显著增加了小梁面积（Tb.Ar%）、小梁厚度（Tb.Th）和小梁数量（Tb.N），并减少了小梁间隔（Tb.Sp）。这些结果表明，染料木黄酮可以抑制乳腺癌溶骨性骨转移，抑制骨吸收，增加骨量并改善骨转移中的骨微结构。