State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqin Rd 18, Haidian District, PO Box 2871, Beijing 100085, China.
Environ Toxicol Pharmacol. 2011 Jan;31(1):189-97. doi: 10.1016/j.etap.2010.10.007. Epub 2010 Nov 5.
In this study, the expression time and profiles of thyroid hormone receptor alpha (trα), type I and II deiodinase enzymes (d1 and d2), transthyretin (ttr), sodium iodide symporter (nis), and thyrotropin-releasing hormone receptor (trhr) genes in Chinese rare minnow (Gobiocypris rarus) were determined using real-time PCR. Meanwhile, the changes of these genes were investigated by exogenous T(3) (3.8 nM) in larvae and adult fish. The retardation of swim bladder development and growth inhibition were observed for larvae, and the transcription of trα, d1, d2, nis, and trhr was significantly down-regulated at the end of exposure (21 d). In adults, a down-regulation of trα, d1, nis, and trhr mRNA levels occurred at 7th or 14th day of exposure, but returned back to their normal levels similar to control at the end of exposure. The down-regulation of gene mRNA expression could serve as a compensatory mechanism for the activation of thyroid system.
本研究采用实时定量 PCR 技术，检测了中国稀有鮈鲫甲状腺激素受体α（trα）、Ⅰ型和Ⅱ型脱碘酶（d1 和 d2）、转甲状腺素蛋白（ttr）、钠碘同向转运体（nis）和促甲状腺素释放激素受体（trhr）基因的表达时间和表达谱。同时，研究了外源性 T3（3.8 nM）对仔鱼和成年鱼上述基因的影响。结果表明，仔鱼出现鳔发育迟缓及生长抑制，暴露结束时（21 天），trα、d1、d2、nis 和 trhr 的转录显著下调。在成年鱼中，7 天或 14 天暴露时 trα、d1、nis 和 trhr 的 mRNA 水平下调，但暴露结束时恢复到与对照组相似的正常水平。基因 mRNA 表达的下调可能是甲状腺系统激活的代偿机制。
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