蛋白质组学生物标志物用于单核细胞-巨噬细胞分化。
Proteomic biosignatures for monocyte-macrophage differentiation.
机构信息
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5880, USA.
出版信息
Cell Immunol. 2011;271(2):239-55. doi: 10.1016/j.cellimm.2011.07.002. Epub 2011 Jul 8.
Abstract
We used pulsed stable isotope labeling of amino acids in cell culture (pSILAC) to assess protein dynamics during monocyte-macrophage differentiation. pSILAC allows metabolic labeling of newly synthesized proteins. Such de novo protein production was evaluated from 3 to 7 days in culture. Proteins were identified by liquid chromatography-tandem mass spectrometry then quantified by MaxQuant. Protein-protein linkages were then assessed by Ingenuity Pathway Analysis. Proteins identified were linked to cell homeostasis, free radical scavenging, molecular protein transport, carbohydrate metabolism, small molecule chemistry, and cell morphology. The data demonstrates specific biologic events that are linked to monocyte transformation in a defined biologic system.
摘要
我们使用脉冲稳定同位素标记的细胞培养中的氨基酸（pSILAC）来评估单核细胞-巨噬细胞分化过程中的蛋白质动态变化。pSILAC 允许对新合成的蛋白质进行代谢标记。从培养的第 3 天到第 7 天评估这种从头蛋白质产生。通过液相色谱-串联质谱法鉴定蛋白质，然后用 MaxQuant 定量。然后通过 Ingenuity Pathway Analysis 评估蛋白质-蛋白质连接。鉴定出的蛋白质与细胞内稳态、自由基清除、分子蛋白转运、碳水化合物代谢、小分子化学和细胞形态有关。这些数据表明，在一个明确的生物系统中，与单核细胞转化相关的特定生物学事件。
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