Panteghini M, Bonora R, Pagani F
Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
Clin Chem. 1990 Mar;36(3):550-3.
A new commercial kit (Impres-MB; International Immunoassay Labs.) recently was introduced for measuring the MB isoenzyme of creatine kinase (CK-MB) based on the use of monoclonal antibodies. After antibodies to CK-MM isoenzyme are added to precipitate the CK-MM, antibodies to CK-M monomer are added to precipitate the M-subunit isoenzymes of CK. Subtracting the enzymatic activity of the second supernate from the residual activity in the first yields the activity of CK-MB. Results are not affected by CK-BB, mitochondrial CK, or adenylate kinase. However, the anti-CK-MM antibodies precipitated only about 98% of serum CK-MM and may have partly precipitated CK-MB isoenzyme (average analytical recovery of CK-MB, 86.6%). Comparison between Impres-MB (y) and electrophoresis (x) yielded the following linear-regression equation: y = 0.79x + 3 (r = 0.982, n = 97). Data for CK-MB temporal kinetics, obtained from patients with myocardial infarction, correlated significantly in both methods; however, peak activity values of CK-MB were significantly different, confirming that the difference between the new method and the electrophoretic method average 20%.
最近推出了一种新的商业试剂盒（Impres-MB；国际免疫分析实验室），用于基于单克隆抗体测量肌酸激酶（CK-MB）的MB同工酶。向样品中加入抗CK-MM同工酶的抗体以沉淀CK-MM，然后加入抗CK-M单体的抗体以沉淀CK的M亚基同工酶。用第一次上清液中的残留活性减去第二次上清液中的酶活性，即可得到CK-MB的活性。结果不受CK-BB、线粒体CK或腺苷酸激酶的影响。然而，抗CK-MM抗体仅沉淀了约98%的血清CK-MM，并且可能部分沉淀了CK-MB同工酶（CK-MB的平均分析回收率为86.6%）。Impres-MB法（y）与电泳法（x）的比较得出以下线性回归方程：y = 0.79x + 3（r = 0.982，n = 97）。从心肌梗死患者获得的CK-MB时间动力学数据，在两种方法中均具有显著相关性；然而，CK-MB的峰值活性值存在显著差异，证实新方法与电泳法之间的差异平均为20%。
