胃癌细胞系SGC - 7901中具有干细胞特性的悬浮细胞。
Floating cells with stem cell properties in gastric cell line SGC-7901.
作者信息
Zheng QiuHong, Gong FuSheng, Xu YangMei, Zheng TianRong, Ying MinGang
机构信息
Laboratory of Surgery, Fujian Provincial Tumor Hospital, Fuzhou, China.
出版信息
Tumori. 2011 May-Jun;97(3):393-9. doi: 10.1177/030089161109700323.
AIMS
To obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres.
METHODS
Serum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres.
RESULTS
A subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins.
CONCLUSIONS
We successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line.
目的
从贴壁生长的胃癌细胞系SGC - 7901中获得悬浮球，并分析这些球体的特性。
方法
使用无血清培养基培养SGC - 7901细胞。采用有限稀释法、肿瘤形成试验、基因芯片分析和实时荧光定量PCR检测球体的干细胞特性。
结果
SGC - 7901细胞的一个亚群在无血清培养基中形成了悬浮球。这些细胞显示出增强的致瘤能力，并且还高表达某些与干细胞相关的蛋白。
结论
我们成功地在SGC - 7901胃癌细胞系中培养出了具有干细胞特性的悬浮球。
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