Health Science Programme, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
Clinics (Sao Paulo). 2011;66(5):753-7. doi: 10.1590/s1807-59322011000500008.
Gingiva fibromatosis is a relatively rare condition characterized by diffuse enlargement of the gingiva, which is caused by expansion and accumulation of the connective tissue.
The aim of the present study was to investigate proliferative and apoptotic biomarker expression in normal gingiva and two forms of gingival fibromatosis.
Archived tissue specimens of hereditary gingival fibromatosis, gingival fibromatosis and dental abnormality syndrome and normal gingiva were subject to morphological analysis and immunohistochemical staining. The results were analyzed statistically.
Proteins associated with proliferation were found in the nuclei of epithelial cells from the basal and suprabasal layers, whereas apoptotic proteins were detected in the cytoplasm of the upper layers of the epithelium. Increased expressions of minichromosome maintenance proteins 2 and 5 were observed in the gingival fibromatosis and dental abnormality syndrome samples. In contrast, geminin expression was higher in normal gingiva samples. No difference in the expression of apoptotic proteins was observed among the groups.
Our findings support a role for augmented proliferation of epithelial cells within the overgrown tissues associated with gingival fibromatosis or dental abnormality syndrome. However, our data suggest that different biological mechanisms may account for the pathogenesis of different types of gingival fibromatosis.
牙龈纤维瘤病是一种相对罕见的疾病，其特征为牙龈弥漫性肿大，这是由结缔组织的扩张和积累引起的。
本研究旨在研究正常牙龈和两种类型的牙龈纤维瘤病中增殖和凋亡生物标志物的表达。
对遗传性牙龈纤维瘤病、牙龈纤维瘤病和牙齿异常综合征以及正常牙龈的存档组织标本进行形态分析和免疫组织化学染色。对结果进行统计学分析。
在基底和超基底上皮细胞的细胞核中发现了与增殖相关的蛋白，而凋亡蛋白则在上皮细胞的上层细胞质中被检测到。在牙龈纤维瘤病和牙齿异常综合征样本中观察到微染色体维持蛋白 2 和 5 的表达增加。相比之下，在正常牙龈样本中，geminin 的表达更高。各组之间的凋亡蛋白表达无差异。
我们的研究结果支持在与牙龈纤维瘤病或牙齿异常综合征相关的过度生长组织中，上皮细胞增殖增加的作用。然而，我们的数据表明，不同类型的牙龈纤维瘤病可能存在不同的发病机制。
