Abe Shin-ichi, Suzuki Masashi, Cho Kwang Ho, Murakami Gen, Cho Baik Hwan, Ide Yoshinobu
Oral Health Science Center HRC8, Tokyo Dental College, Chiba, Japan.
Surg Radiol Anat. 2011 Dec;33(10):919-27. doi: 10.1007/s00276-011-0854-2. Epub 2011 Jul 26.
CD34 is a well-known marker of progenitor cells of blood vessels and stromal tissues. Thus, CD34-positive cells have recently been used clinically in the field of vascular and orthopedic biotechnology because of their capacity to assist regeneration of injured tissues. However, to our knowledge, the in situ distribution of CD34-positive cells has not yet been described in the human fetus, with the exception of a few organs. In the present study, we conducted immunohistochemistry for CD34 using 12 human fetuses (9-15 weeks of gestation). CD34-positive structures showed a vessel-like appearance and were regularly arrayed in the viscera, nerves and lymph nodes, whereas in the body wall and extremities, they were distributed diffusely as fibrous tissues, such as the fascia and perimysium. The myocardium was also divided and bundled by CD34-positive fibrous tissues. In striated muscles, limited examples of CD34 expression were found in the tongue and extraocular muscles in which only vessels were positive. Lymphatic vessels were negative for CD34. In addition to their contribution to vascular development in any part of the body, CD34-positive mesenchymal tissues seem to play a critical role in the pattern formation of skeletal muscle, synovial tissue and other muscle/tendon-associated tissues in human fetuses.
CD34是血管和基质组织祖细胞的一种著名标志物。因此，CD34阳性细胞因其具有协助受损组织再生的能力，最近已在血管和骨科生物技术领域得到临床应用。然而，据我们所知，除少数器官外，人类胎儿中CD34阳性细胞的原位分布尚未见报道。在本研究中，我们使用12例人类胎儿（妊娠9 - 15周）进行了CD34免疫组织化学检测。CD34阳性结构呈血管样外观，在内脏、神经和淋巴结中呈规则排列，而在体壁和四肢，它们作为纤维组织（如筋膜和肌周膜）呈弥漫性分布。心肌也被CD34阳性纤维组织分隔和束状排列。在横纹肌中，仅在舌肌和眼外肌中发现有限的CD34表达实例，其中只有血管呈阳性。淋巴管对CD34呈阴性。除了对身体任何部位的血管发育有贡献外，CD34阳性间充质组织似乎在人类胎儿骨骼肌、滑膜组织和其他肌肉/肌腱相关组织的模式形成中起关键作用。
