裂殖酵母粟酒裂殖酵母的腺苷酸环化酶活性不受鸟苷核苷酸的调节。

Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides.

作者信息

Engelberg D, Poradosu E, Simchen G, Levitzki A

机构信息

Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.

出版信息

FEBS Lett. 1990 Feb 26;261(2):413-8. doi: 10.1016/0014-5793(90)80605-i.

DOI:10.1016/0014-5793(90)80605-i

PMID:2178979

Abstract

The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 +/- 0.14.

摘要

裂殖酵母粟酒裂殖酵母的腺苷酸环化酶活性定位于细胞的质膜。该酶利用Mn2+/ATP作为底物，游离的Mn2+离子作为效应物。与酿酒酵母不同，粟酒裂殖酵母的腺苷酸环化酶不利用Mg2+/ATP作为底物，其活性也不受鸟苷核苷酸的刺激。粟酒裂殖酵母腺苷酸环化酶活性的最适pH值为6.0。其活性对ATP的依赖性具有协同性，希尔系数为1.68±0.14。