[基于GeXP多重PCR检测法的人乳头瘤病毒检测与分型]
[Detection and typing of human papillomavirus by a GeXP based multiplex PCR assay].
作者信息
Lu Chun-bin, Yang Meng-jie, Luo Le, Wang Miao, Ma Xue-jun
机构信息
Institute of Reproductive Immunology, JiNan University, Guangzhou 510632, China
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Feb;25(1):69-72.
OBJECTIVE
To establish a new and rapid GeXP based multiplex PCR assay for the detection and typing of human papillomavirus 6, 11, 31, 33 and 52.
METHODS
Nucleotide sequences of HPV6, HPV11, HPV31, HPV33 and HPV52 from NCBI were obtained and compared. Genotype-specific primers were then designed and the sensitivity and specificity of multiple PCR assay was evaluated. Optimized assay was further validated with 30 clinical specimens collected from the cervical secretions of patients.
RESULTS
A GeXP based multiplex PCR was developed for sensitive detection and reliable differentiation of five HPV genotypes (HPV6, 11, 31, 33 and 52),
CONCLUSION
A GeXP based multiplex PCR assay is demonstrated to be a new and rapid technique for simultaneous detection and typing of 5 different human papillomaviruses.
目的
建立一种基于GeXP的新型快速多重PCR检测方法，用于检测人乳头瘤病毒6型、11型、31型、33型和52型并进行基因分型。
方法
从NCBI获取HPV6、HPV11、HPV31、HPV33和HPV52的核苷酸序列并进行比较。随后设计基因型特异性引物，并评估多重PCR检测方法的敏感性和特异性。采用从患者宫颈分泌物中收集的30份临床标本对优化后的检测方法进行进一步验证。
结果
开发了一种基于GeXP的多重PCR方法，用于灵敏检测和可靠区分5种HPV基因型（HPV6、11、31、33和52）。
结论
基于GeXP的多重PCR检测方法被证明是一种用于同时检测5种不同人乳头瘤病毒并进行基因分型的新型快速技术。
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