Vershinina Z P, Baĭmiev An Kh, Blagova D K, Kniazev A V, Baĭmiev Al Kh, Chemeris A V
Prikl Biokhim Mikrobiol. 2011 May-Jun;47(3):336-42.
"Barbate roots" in tobacco and colza transgenic on lectin gene were obtained with the use of a wild strain of Agrobacterium rhizogenes 15834 transformed with pCAMBIA1305.1 plasmid containing the full-size lectin gene (psl) from the Pisum sativum. Influence of expression oflectin gene on colonization oftransgenic roots with symbiont of pea (Rhizobium leguminosarum) was investigated. The number of adhered bacteria onto the roots transformed with lectin gene was 14-fold and 37-fold higher in comparison with the control; this confirms the interaction of R. leguminosarum with pea lectin at the surface of the transformed roots of tobacco and colza. The developed experimental approach, based on the simulation of recognition processes and early symbiotic interactions with lectins of pea plants, may, in perspective, be used for obtaining stable associations of economically valuable, nonsymbiotrophic plant species with rhizobia.
利用用含来自豌豆（Pisum sativum）的全长凝集素基因（psl）的pCAMBIA1305.1质粒转化的发根农杆菌（Agrobacterium rhizogenes）15834野生菌株，获得了烟草和油菜中凝集素基因转基因的毛状根。研究了凝集素基因表达对豌豆共生菌（根瘤菌Rhizobium leguminosarum）在转基因根上定殖的影响。与对照相比，用凝集素基因转化的根上附着的细菌数量高14倍和37倍；这证实了根瘤菌与烟草和油菜转化根表面的豌豆凝集素之间的相互作用。基于模拟识别过程和与豌豆植物凝集素的早期共生相互作用所开发的实验方法，从长远来看，可用于获得经济价值高的非共生营养植物物种与根瘤菌的稳定关联。
