Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, Medical School, University of Crete, GR-71110 Heraklion, Greece.
J Proteome Res. 2011 Sep 2;10(9):4241-51. doi: 10.1021/pr200422f. Epub 2011 Aug 15.
The interaction between the immune system and invading bacteria is sufficient to eradicate microorganisms for the majority of bacterial infections, but suppression of the microbicidal response leads to reactivation or chronic evolution of infections and to bacterial persistence. To identify the cellular pathways affected by bacterial persistence, we applied the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique for a comparative study of protein expression in the C. burnetii strains Nine Mile (NM) and its respective strain (NMper) isolated from 18 months persistently infected cell cultures. In total, three different proteome comparisons were performed with the total bacterial proteome, potentially secreted bacterial proteins, and the eukaryotic infected proteome being assessed. Our results revealed that among the 547 identified bacterial proteins, 53 had significantly altered levels of expression and indicated potential metabolic differences between the two strains. Regarding differences in the secreted proteins between both strains and different modulation of the host cell, machineries reflect at least large rearrangements of both bacterial and eukaryotic proteomes during the persistent model of infection when compared to the acute one, which emphasizes that C. burnetii orchestrates a vast number of different bacterial and eukaryotic host cell processes to persist within its host.
免疫系统与入侵细菌的相互作用足以消除大多数细菌感染的微生物，但微生物杀灭反应的抑制会导致感染的重新激活或慢性演变以及细菌的持续存在。为了确定受细菌持续存在影响的细胞途径，我们应用了基于 MS 的联合分数对角色谱（COFRADIC）蛋白质组学技术，对从持续感染 18 个月的细胞培养物中分离出的九英里（NM）菌株及其相应菌株（NMper）的蛋白质表达进行了比较研究。总共进行了三次不同的全细菌蛋白质组、潜在分泌细菌蛋白质和受感染真核细胞蛋白质组的比较研究。我们的结果表明，在鉴定出的 547 种细菌蛋白质中，有 53 种的表达水平明显改变，表明这两种菌株之间可能存在代谢差异。关于两种菌株之间分泌蛋白的差异和宿主细胞的不同调节，这些机制至少反映了在持续感染模型中与急性感染模型相比，细菌和真核细胞蛋白质组的大规模重组，这强调了伯氏考克斯体在其宿主内持续存在时会协调大量不同的细菌和真核宿主细胞过程。
