Song Tao, Zhang Xu, Wang Chunyang, Wu Yiguang, Dong Jun, Gao Jiangping, Cai Wei, Hong Baofa
Department of Urology, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing, China.
Asian Pac J Cancer Prev. 2011;12(4):929-33.
Systemic chemotherapy is the only current modality that provides the potential for long-term survival in bladder carcinoma patients with metastatic disease. Overexpression of cyclooxygenase-2 COX-2 induces expression of immune- and cell proliferation-related genes and is associated with the grade, prognosis and recurrence of transitional cell carcinoma of the bladder. There is abundant evidence that aberrant expression of microRNAs (miRNAs) is implicated in numerous disease states and miRNAs have the potential to be used for cancer therapeutics. Here, we found expression of miR-143 to be low in a series of human bladder carcinomas as compared to background tissue. In addition, restoration of miR-143 by cell transfection in T24 cancer cells led to decreased COX-2 expression, reduced proliferation and mobility. Our findings will help to further understand the functions of miRNAs in cancer cells and point to a specific potential of miR-143 may be employed as a therapeutic agent for bladder carcinoma. The results provide insights into the development of novel tumor markers or new therapeutic strategies.
全身化疗是目前唯一有可能让转移性膀胱癌患者获得长期生存的治疗方式。环氧合酶-2（COX-2）的过表达可诱导免疫和细胞增殖相关基因的表达，并与膀胱移行细胞癌的分级、预后及复发相关。有充分证据表明，微小RNA（miRNA）的异常表达与多种疾病状态有关，且miRNA有潜力用于癌症治疗。在此，我们发现与背景组织相比，一系列人类膀胱癌中miR-143的表达较低。此外，通过细胞转染在T24癌细胞中恢复miR-143的表达会导致COX-2表达降低、增殖和迁移能力减弱。我们的研究结果将有助于进一步了解miRNA在癌细胞中的功能，并指出miR-143可能具有作为膀胱癌治疗药物的特定潜力。这些结果为新型肿瘤标志物的开发或新的治疗策略提供了见解。
