Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
Nat Prod Res. 2012;26(10):933-8. doi: 10.1080/14786419.2010.534999. Epub 2011 Jul 27.
Cisplatin is an anticancer drug extensively used against a variety of cancers. Cisplatin chemotherapy is found to manifest dose-dependent nephrotoxicity. Depletion of the renal antioxidant defence system has been suggested to be the main cause of cisplatin-induced nephrotoxicity. The purpose of this study is to investigate whether the ethanol extract of entire plant of Sphaeranthus indicus could reduce the intensity of toxicity in albino rats. Nephrotoxicity was assessed by determining the serum creatinine and urea levels and renal antioxidant status in rats after cisplatin administration (12 mg kg(-1) body weight, i.p.). The ethanol extract of S. indicus (150 and 300 mg kg(-1) body weight) was administered orally from the sixth day onwards for 10 days after cisplatin administration. The extract significantly reduced the elevated serum creatinine and urea levels. Renal antioxidant defence systems, such as superoxide dismutase, catalase, glutathione peroxidase activities and reduced glutathione level that are depleted by cisplatin therapy were restored to normal by treatment with the extract. Cisplatin-induced lipid peroxidation was also found to be markedly reduced by treatment with the extract. These results suggest that S. indicus has protective effect against cisplatin-induced nephrotoxicity, which may be attributed to its antioxidant potential.
顺铂是一种广泛用于多种癌症的抗癌药物。顺铂化疗被发现表现出剂量依赖性肾毒性。据认为，肾抗氧化防御系统的耗竭是顺铂诱导肾毒性的主要原因。本研究旨在探讨印度千日红全草的乙醇提取物是否可以降低白化大鼠的毒性强度。通过测定顺铂给药后（12mg/kg 体重，ip）大鼠的血清肌酐和尿素水平以及肾抗氧化状态来评估肾毒性。顺铂给药后，从第 6 天开始，每天口服给予 S.indicus 乙醇提取物（150 和 300mg/kg 体重）10 天。提取物显著降低了升高的血清肌酐和尿素水平。顺铂治疗耗竭的肾抗氧化防御系统，如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性和还原型谷胱甘肽水平，通过用提取物处理恢复正常。顺铂诱导的脂质过氧化也被发现通过用提取物处理而明显减少。这些结果表明，S.indicus 对顺铂诱导的肾毒性具有保护作用，这可能归因于其抗氧化潜力。
