Etiz Sayharman Sema, Tosun Nihan, Aka Nurettin, Köse Gültekin, Tekin Ali, Gören Zafer
Obstetrics and Gynecology Clinic, Haydarpaşa Numune Training and Research Hospital, Duzce, Turkey.
J Obstet Gynaecol Res. 2011 Nov;37(11):1638-44. doi: 10.1111/j.1447-0756.2011.01589.x. Epub 2011 Jul 25.
The aim of this study was to investigate the effects of estrogen plus progesterone treatment administered after surgical menopause on morphological and muscarinic receptor sensitivity in detrusor muscle bands of rats.
A total of 40 female Albino-Wistar rats were assigned into two groups to undergo surgical menopause with sham operation (n = 10) and bilateral oophorectomy (n = 30). Thirty oophorectomized rats were assigned into three groups for different treatments administered for 8 weeks beginning from the 7th postoperative day. At the end of the 8th postoperative week, laparotomy was performed in all of them and the urinary bladders were excised and investigated with light microscope. All statistical analysis and graphs were performed using GraphPad Prism version 4. P < 0.05 was considered to be statistically significant.
It was determined that average bodyweights were increased to some extent in oophorectomized and sham groups after treatment and while it was more marked in the group receiving estrogen therapy, bodyweights were decreased in the group receiving estrogen plus progesterone therapy. However, this was not statistically significant. No statistically significant difference was determined between the sham, placebo and 17-β estradiol treatment groups regarding contractile response of urinary bladder tapes to carbachol (P > 0.05). Contractile responses of urinary bladder tapes of the estrogen plus progesterone treatment group were found to be statistically lower than the placebo group (P < 0.05).
Our in vitro findings demonstrate that treatment with estrogen plus progesterone decreases muscarinic activity in oophorectomized rats, whereas estrogen-only treatment does not. Our study establishes the basis for further studies to answer whether combination of estrogen and progesterone treatment can restore detrusor overactivity associated with sex hormone defects seen at menopause.
本研究旨在探讨手术绝经后给予雌激素加孕激素治疗对大鼠逼尿肌条形态和毒蕈碱受体敏感性的影响。
总共40只雌性白化Wistar大鼠被分为两组，分别接受假手术（n = 10）和双侧卵巢切除术（n = 30）以实现手术绝经。30只接受卵巢切除术的大鼠从术后第7天开始被分为三组进行不同治疗，为期8周。术后第8周结束时，对所有大鼠进行剖腹手术，切除膀胱并进行光学显微镜检查。所有统计分析和图表均使用GraphPad Prism 4版本完成。P < 0.05被认为具有统计学意义。
确定治疗后去卵巢组和假手术组的平均体重均有一定程度增加，虽然接受雌激素治疗的组体重增加更为明显，但接受雌激素加孕激素治疗的组体重下降。然而，这并无统计学意义。在假手术组、安慰剂组和17-β雌二醇治疗组之间，膀胱条带对卡巴胆碱的收缩反应未发现统计学显著差异（P > 0.05）。雌激素加孕激素治疗组膀胱条带的收缩反应在统计学上低于安慰剂组（P < 0.05）。
我们的体外研究结果表明，雌激素加孕激素治疗可降低去卵巢大鼠的毒蕈碱活性，而仅用雌激素治疗则无此效果。我们的研究为进一步研究雌激素和孕激素联合治疗能否恢复绝经时出现的与性激素缺陷相关的逼尿肌过度活动奠定了基础。
