Production of toxic enzymes of Clostridium perfringens BP6K on technologically important media including a medium with Tween 80.

The addition of 0.05--0.1% of Tween 80 to casein hydrolysate medium of Adams and Hendee raises and production of Clostridium perfringens BP6K lecithinase by approximately 50%. The use of the silicon antifoam Lukosan makes the medium particularly suitable for cultivation in large volumes. Higher levels of theta haemolysin and lecithinase are formed on the casein hydrolysate medium than on the meat medium. All factors except the theta and lambda components are farily stable during cultivation.