鸡滑液囊支原体日本田间分离株的基因分型及与MS-H疫苗株的快速分子鉴别

Genotyping of Japanese field isolates of Mycoplasma synoviae and rapid molecular differentiation from the MS-H vaccine strain.

作者信息

Ogino Shoji, Munakata Yasuhisa, Ohashi Shuichi, Fukui Masato, Sakamoto Hiroshi, Sekiya Yukio, Noormohammadi Amir H, Morrow Chris J

机构信息

Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd., Asaka-machi, Koriyama-shi, Fukushima 963-0196, Japan.

出版信息

Avian Dis. 2011 Jun;55(2):187-94. doi: 10.1637/9461-071310-Reg.1.

DOI:10.1637/9461-071310-Reg.1

PMID:21793432

Abstract

Mycoplasma synoviae is an important causative agent of avian mycoplasmosis. In the present study the conserved domain of the variable lipoprotein and hemagglutinin (vlhA) gene of M. synoviae was sequenced and analyzed for 19 field strains of M. synoviae isolated from chickens across Japan. This analysis revealed that there were at least nine genotypes of M. synoviae present in Japan. Furthermore, we found a single nucleotide polymorphism (SNP) within this region in all the Japanese isolates, and based on this finding, we established a PCR method with cycling probe technology to differentiate between these field isolates and the live M. synoviae vaccine strain Mycoplasma synoviae-H (MS-H).

摘要

滑膜支原体是禽支原体病的重要病原体。在本研究中，对从日本各地鸡群中分离出的19株滑膜支原体田间菌株的可变脂蛋白和血凝素（vlhA）基因的保守结构域进行了测序和分析。该分析表明，日本存在至少九种滑膜支原体基因型。此外，我们在所有日本分离株的该区域内发现了一个单核苷酸多态性（SNP），基于这一发现，我们建立了一种采用循环探针技术的PCR方法，以区分这些田间分离株和活的滑膜支原体疫苗株滑膜支原体-H（MS-H）。