Cizelj I, Berčič R L, Slavec B, Narat M, Dovč P, Benčina D
a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
b Veterinary Faculty , University of Ljubljana , Ljubljana , Slovenia.
Br Poult Sci. 2015;56(6):658-65. doi: 10.1080/00071668.2015.1113506.
The aim of this study was to identify molecular techniques which enable clear discrimination between Mycoplasma synoviae isolates for improved epidemiology. Multilocus sequence analysis (MLSA) of 6 M. synoviae loci was conducted for genotyping of isolates with previously determined 5'-vlhA sequences. Sequencing of three polymorphic genes (5'-vlhA, cysP and nanH) enables good discrimination between isolates with different genotypes. Such a genotyping scheme revealed 10 distinct genotypes, which were confirmed by sequencing of an additional three loci of the M. synoviae genome. Epidemiologically linked strains formed clusters with the same genotypes which clearly differed between clusters. MLSA used in this study is a promising tool for epidemiology of M. synoviae isolates, but it should be evaluated by further investigations using a much higher number of M. synoviae strains.
本研究的目的是确定能够清晰区分滑膜支原体分离株的分子技术，以改进流行病学研究。对6个滑膜支原体基因座进行多位点序列分析（MLSA），用于对具有先前确定的5'-vlhA序列的分离株进行基因分型。对三个多态性基因（5'-vlhA、cysP和nanH）进行测序能够很好地区分不同基因型的分离株。这样的基因分型方案揭示了10种不同的基因型，这通过对滑膜支原体基因组另外三个基因座的测序得到了证实。具有流行病学关联的菌株形成了具有相同基因型的簇，不同簇之间明显不同。本研究中使用的MLSA是用于滑膜支原体分离株流行病学研究的一种有前景的工具，但应该通过使用更多数量的滑膜支原体菌株进行进一步研究来对其进行评估。
