Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
Anal Chem. 2011 Sep 1;83(17):6778-84. doi: 10.1021/ac201462d. Epub 2011 Aug 9.
Magnetic Fe(3)O(4) particle aggregates were prepared by cross-linking Fe(3)O(4) nanoparticles bearing surface carbonyl groups with poly-L-lysine. Upon further coupling with antiparaoxon methyl polyclonal antibody, the resultant particle aggregate-based probes were used in a lateral flow immunochromatographic assay (LFIA) of pesticide residue of paraoxon methyl. The results were compared with that achieved by using the mother Fe(3)O(4) nanoparticles. More quantitative results on the signal amplification effect endowed by the controlled aggregation of Fe(3)O(4) nanoparticles were extracted by relative optical density analysis. Under optimized conditions, a detection limit of 1.7 ng/mL for paraoxon methyl was achieved by using the particle aggregates, which is almost 40-fold lower than that based on the mother Fe(3)O(4) nanoparticles.
磁性 Fe(3)O(4) 颗粒聚集体是通过交联具有表面羰基的 Fe(3)O(4)纳米粒子与聚-L-赖氨酸来制备的。在与抗对氧磷甲基多克隆抗体进一步偶联后，所得基于颗粒聚集体的探针用于对氧磷甲基农药残留的侧向流动免疫层析分析 (LFIA)。结果与使用母体 Fe(3)O(4)纳米粒子的结果进行了比较。通过相对光密度分析，提取了由 Fe(3)O(4)纳米粒子的受控聚集赋予的信号放大效果的更定量的结果。在优化条件下，使用颗粒聚集体可以实现对氧磷甲基的检测限为 1.7ng/mL，比基于母体 Fe(3)O(4)纳米粒子的检测限低近 40 倍。
