Morhenn V B, Kaye J A
J Invest Dermatol. 1979 Mar;72(3):138-42. doi: 10.1111/1523-1747.ep12530603.
Pre-treatment of baby hamster kidney (BHK) cells with 8-methoxypsoralen (8-MOP) plus ultraviolet (UV) light enhances the frequency of their transformation by polyoma (Py) virus. Of the doses tested, 0.5 microgram/ml 8-MOP plus 0.3 J/cm2 UV-light results in maximal (30-fold) stimulation of viral transformation. 8-MOP alone does not affect viral transformation and UV-light alone causes only a slight increase in the transformation frequency. Thus the drug and light act synergistically in promoting the effect. Treatment of BHK cells with drug plus light without Py infection does not lead to a transformed morphology. A drug-light combination (0.01 microgram/ml 8-MOP plus 1.2 J/cm2 UV) that inhibits cellular DNA synthesis to 75% of control at 28 hr after treatment results in a 6-fold stimulation of the transformation frequency.
用8-甲氧基补骨脂素（8-MOP）加紫外线（UV）对幼仓鼠肾（BHK）细胞进行预处理，可提高多瘤（Py）病毒使其发生转化的频率。在所测试的剂量中，0.5微克/毫升8-MOP加0.3焦/平方厘米紫外线可使病毒转化受到最大程度（30倍）的刺激。单独使用8-MOP不影响病毒转化，单独使用紫外线仅使转化频率略有增加。因此，药物和光线在促进该效应方面具有协同作用。用药物加光线处理BHK细胞但不感染Py病毒，不会导致细胞形态发生转化。一种药物-光线组合（0.01微克/毫升8-MOP加1.2焦/平方厘米紫外线）在处理后28小时可将细胞DNA合成抑制至对照的75%，该组合可使转化频率提高6倍。
