Luoga W, Mansur F, Buttle D J, Duce I R, Garnett M C, Behnke J M
School of Biology, University of Nottingham, University Park, Nottingham, UK.
J Helminthol. 2012 Sep;86(3):311-6. doi: 10.1017/S0022149X11000368. Epub 2011 Jul 28.
In earlier studies of the anthelmintic activity of plant cysteine proteinases (CPs), a period of food deprivation was routinely employed before administration of CPs, but there has been no systematic evaluation as to whether this does actually benefit the anthelmintic efficacy. Therefore, we assessed the effect of fasting on the efficacy of CPs from papaya latex (PL) against Heligmosomoides bakeri in C3H mice. We used a refined, supernatant extract of papaya latex (PLS) with known active enzyme content. The animals were divided into three groups (fasted prior to treatment with PLS, not fasted but treated with PLS and fasted but given only water). The study demonstrated clearly that although food deprivation had been routinely employed in much of the earlier work on CPs in mice infected with nematodes, fasting has no beneficial effect on the efficacy of PLS against H. bakeri infections. Administration of CPs to fed animals will also reduce the stress associated with fasting.
在早期关于植物半胱氨酸蛋白酶（CPs）驱虫活性的研究中，在给予CPs之前通常会有一段禁食期，但对于这样做是否真的有利于驱虫效果，尚无系统评估。因此，我们评估了禁食对木瓜乳胶（PL）中的CPs抗C3H小鼠体内巴氏赫勒线虫效力的影响。我们使用了一种精制的、已知活性酶含量的木瓜乳胶上清提取物（PLS）。将动物分为三组（用PLS处理前禁食、未禁食但用PLS处理以及禁食但只给水）。该研究清楚地表明，尽管在许多早期关于感染线虫小鼠的CPs研究中常规采用了禁食，但禁食对PLS抗巴氏赫勒线虫感染的效力没有有益影响。给进食的动物施用CPs也将减少与禁食相关的应激。
