IPICyT, Instituto Potosino de Investigación Científica y Tecnología, Camino a la Presa San José No. 2055, Lomas 4a sección, 78216 San Luis Potosí, SLP, Mexico.
J Plant Physiol. 2011 Nov 15;168(17):2102-9. doi: 10.1016/j.jplph.2011.06.006. Epub 2011 Jul 26.
Calcium (Ca(2+)) is a critical ion for the growth and development of plants and plays an important role in signal transduction pathways in response to biotic and abiotic stresses. We investigated the Ca(2+) stress responsive-genes in amaranth leaves by using the suppression subtractive hybridization technique. Screening of the libraries generated 420 up-regulated transcripts and 199 down-regulated transcripts. The differentially expressed transcripts were associated with general stress response, transcription factors, gene regulation, signal transduction, and some other with unknown function. Selected genes were used to study their differential regulation by sqRT-PCR. Among the up-regulated transcripts, a fragment containing the motif of C3HC4-type RING-Zinc family was further characterized. The ORF of amaranth zinc finger protein (AhZnf) has a closer relationship with its ortholog from Ricinus communis while is distantly related to the Arabidopsis thaliana C3HC4-type ortholog. We have identified a novel putative zinc finger protein along with other novel proteins such as the wall associated kinase, phosphoinositide binding protein, and rhomboid protease involved in response to Ca(2+) stress in amaranth leaves.
钙（Ca(2+)) 是植物生长和发育的关键离子，在生物和非生物胁迫响应的信号转导途径中发挥重要作用。我们使用抑制差减杂交技术研究了苋菜叶片中的 Ca(2+) 应激响应基因。对文库的筛选产生了 420 个上调转录本和 199 个下调转录本。差异表达的转录本与一般应激反应、转录因子、基因调控、信号转导有关，还有一些具有未知功能。选择了一些基因进行 sqRT-PCR 研究其差异调控。在上调的转录本中，进一步鉴定出一个含有 C3HC4 型 RING-Zinc 家族基序的片段。苋菜锌指蛋白（AhZnf）的 ORF 与其来自蓖麻的同源物具有更密切的关系，而与拟南芥的 C3HC4 型同源物关系较远。我们已经鉴定出一种新型的锌指蛋白，以及其他新型蛋白，如参与苋菜叶片 Ca(2+) 应激反应的壁相关激酶、磷酸肌醇结合蛋白和类蛋白水解酶。
