Warley A
Division of Biochemistry, U.M.D.S., London, U.K.
J Microsc. 1990 Feb;157(Pt 2):135-47. doi: 10.1111/j.1365-2818.1990.tb02954.x.
A review on the subject of compounds used as standards for biological X-ray microanalysis is presented. The general approach used for standardization has been to use standards which resemble the specimen closely in composition. Thus, standards based on proteins have been used for analysis of quench-frozen cryosectioned specimens, whereas standards based on embedding resins have been used for resin-embedded material. The properties of, and problems associated with, each type of standard are recognized and have been well documented. The choice and analysis of biological material. Attention is drawn to the fact that the problems associated with any quantification procedure need to be kept in mind when analysis of standards is undertaken.
本文对用作生物X射线微分析标准的化合物主题进行了综述。标准化所采用的一般方法是使用在成分上与标本非常相似的标准。因此，基于蛋白质的标准已用于对快速冷冻的冷冻切片标本进行分析，而基于包埋树脂的标准已用于树脂包埋材料。每种标准的特性以及与之相关的问题都已得到认识并有充分记录。生物材料的选择和分析。需要注意的是，在对标准进行分析时，要牢记与任何定量程序相关的问题。
