一种用于保存尿液和膀胱冲洗细胞以对膀胱癌患者进行流式细胞术监测的临床适用方法。

A clinically applicable method to preserve urine and bladder washing cells for flow cytometric monitoring of bladder cancer patients.

作者信息

Deitch A D, Andreotti V A, Strand M A, Howell L, deVere White R W

机构信息

Department of Urology, University of California, Davis, Sacramento.

出版信息

J Urol. 1990 Apr;143(4):700-5. doi: 10.1016/s0022-5347(17)40064-4.

DOI:10.1016/s0022-5347(17)40064-4

PMID:2179581

Abstract

We describe a method to fix exfoliated bladder cells that is suitable for followup of bladder cancer patients by deoxyribonucleic acid flow cytometry. After fixation with room temperature methanol plus acetic acid (20:1, volume:volume) urine and bladder washing samples from these patients can be stored at room temperature for 3 to 7 days and then assessed reliably for the presence of aneuploidy and the percentage of hyperdiploid cells. For those with active transitional cell carcinoma diagnostic accuracy comparing fresh to fixed specimens was improved from 58 to 92% with urine and from 50 to 100% with washing samples. For patients with a history of transitional cell carcinoma who currently are free of disease the false positive rate remains unchanged after fixation. The procedure described is suitable for use in the outpatient clinic and should permit shipping of samples without refrigeration to a central flow cytometry facility for analysis.

摘要

我们描述了一种固定脱落膀胱细胞的方法，该方法适用于通过脱氧核糖核酸流式细胞术对膀胱癌患者进行随访。用室温甲醇加乙酸（20:1，体积比）固定后，这些患者的尿液和膀胱冲洗样本可在室温下保存3至7天，然后可靠地评估非整倍体的存在情况以及超二倍体细胞的百分比。对于患有活动性移行细胞癌的患者，新鲜样本与固定样本相比，尿液样本的诊断准确率从58%提高到92%，冲洗样本的诊断准确率从50%提高到100%。对于有移行细胞癌病史且目前无疾病的患者，固定后假阳性率保持不变。所描述的程序适用于门诊诊所，并且应该允许在不冷藏的情况下将样本运送到中央流式细胞术设施进行分析。