Purification of DNA-binding proteins by site-specific DNA affinity chromatography.

Suppr

超能文献

作者信息

Eisenberg S, Francesconi S C, Civalier C, Walker S S

出版信息

Methods Enzymol. 1990;182:521-9. doi: 10.1016/0076-6879(90)82041-y.

DOI:10.1016/0076-6879(90)82041-y

PMID:2179667

Abstract

摘要

相似文献

Purification of DNA-binding proteins by site-specific DNA affinity chromatography.

Methods Enzymol. 1990;182:521-9. doi: 10.1016/0076-6879(90)82041-y.

Isolation and assay of mitochondrial transcription termination factor from human cells.

Methods Enzymol. 1996;264:158-73. doi: 10.1016/s0076-6879(96)64017-8.

Reconstitution of protein-DNA complexes for crystallization.用于结晶的蛋白质 - DNA 复合物的重构。

Methods Mol Biol. 2001;148:547-56. doi: 10.1385/1-59259-208-2:547.

Purification of heat shock transcription factor of Drosophila.果蝇热休克转录因子的纯化

Methods Enzymol. 1996;274:113-9. doi: 10.1016/s0076-6879(96)74011-9.

Assembly and transcription of chromatin templates using RNA polymerase III.使用RNA聚合酶III进行染色质模板的组装和转录。

Methods Mol Biol. 1995;37:47-66. doi: 10.1385/0-89603-288-4:47.

DNA affinity chromatography.DNA亲和层析法

Methods Enzymol. 1995;254:595-604. doi: 10.1016/0076-6879(95)54042-3.

Identifying DNA replication complex components using protein affinity chromatography.利用蛋白质亲和色谱法鉴定DNA复制复合体成分

Methods Enzymol. 1995;262:415-30. doi: 10.1016/0076-6879(95)62033-8.

Identification and purification of DBF-A, a double-stranded DNA-binding protein from Saccharomyces cerevisiae.酿酒酵母双链DNA结合蛋白DBF-A的鉴定与纯化。

J Biol Chem. 1992 Jan 25;267(3):1648-54.

A new fast and easy method for the oligonucleotide affinity purification of DNA binding proteins.一种用于DNA结合蛋白的寡核苷酸亲和纯化的快速简便新方法。

Nucleic Acids Res. 1991 Jun 11;19(11):3156. doi: 10.1093/nar/19.11.3156.

Purification of sequence-specific binding proteins by DNA affinity chromatography.

Methods Enzymol. 1991;208:10-23. doi: 10.1016/0076-6879(91)08004-2.

引用本文的文献

The Ku-like protein from Saccharomyces cerevisiae is required in vitro for the assembly of a stable multiprotein complex at a eukaryotic origin of replication.

Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11569-74. doi: 10.1073/pnas.93.21.11569.

Insect immunity. Two 17 bp repeats nesting a kappa B-related sequence confer inducibility to the diptericin gene and bind a polypeptide in bacteria-challenged Drosophila.昆虫免疫。两个嵌套κB相关序列的17碱基对重复序列赋予双翅肽基因诱导性，并在受到细菌挑战的果蝇中与一种多肽结合。

EMBO J. 1993 Apr;12(4):1561-8. doi: 10.1002/j.1460-2075.1993.tb05800.x.

Purification and cDNA cloning of a transcription factor which functionally cooperates within a cAMP regulatory unit in the porcine uPA gene.猪尿激酶型纤溶酶原激活剂（uPA）基因中一个在cAMP调节单元内发挥功能协同作用的转录因子的纯化及cDNA克隆

Nucleic Acids Res. 1993 Apr 25;21(8):1845-52. doi: 10.1093/nar/21.8.1845.

Interaction of two sequence-specific single-stranded DNA-binding proteins with an essential region of the beta-casein gene promoter is regulated by lactogenic hormones.两种序列特异性单链DNA结合蛋白与β-酪蛋白基因启动子的一个必需区域之间的相互作用受催乳激素调节。

Mol Cell Biol. 1993 Dec;13(12):7303-10. doi: 10.1128/mcb.13.12.7303-7310.1993.

The maize (Zea mays L.) Cat1 catalase promoter displays differential binding of nuclear proteins isolated from germinated and developing embryos and from embryos grown in the presence and absence of abscisic acid.玉米（Zea mays L.）Cat1过氧化氢酶启动子显示，从萌发和发育中的胚以及在脱落酸存在和不存在的情况下生长的胚中分离出的核蛋白具有不同的结合情况。

Plant Physiol. 1994 Dec;106(4):1373-80. doi: 10.1104/pp.106.4.1373.

DNA polymers of protein binding sequences generated by PCR.通过聚合酶链式反应（PCR）生成的蛋白质结合序列的DNA聚合物。

Nucleic Acids Res. 1990 Nov 11;18(21):6460. doi: 10.1093/nar/18.21.6460.

A DNA replication enhancer in Saccharomyces cerevisiae.酿酒酵母中的一种DNA复制增强子。

Proc Natl Acad Sci U S A. 1990 Jun;87(12):4665-9. doi: 10.1073/pnas.87.12.4665.

Analysis of the interactions of functional domains of a nuclear origin of replication from Saccharomyces cerevisiae.酿酒酵母核复制起点功能域相互作用的分析。

Nucleic Acids Res. 1991 Nov 25;19(22):6255-62. doi: 10.1093/nar/19.22.6255.

At least three distinct proteins are necessary for the reconstitution of a specific multiprotein complex at a eukaryotic chromosomal origin of replication.在真核生物染色体复制起点处重建特定的多蛋白复合体至少需要三种不同的蛋白质。

Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11156-60. doi: 10.1073/pnas.89.23.11156.

Opaque-2 is a transcriptional activator that recognizes a specific target site in 22-kD zein genes.不透明2是一种转录激活因子，可识别22-kD玉米醇溶蛋白基因中的特定靶位点。

Plant Cell. 1992 Jun;4(6):689-700. doi: 10.1105/tpc.4.6.689.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验