Zhang Da-hong, Zhu Wei-guo, Yue Shun
Department of Oncology, Affiliated Huaian First Hospital of Nanjing Medical University, Huaian, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 May;27(5):551-2, 554.
To study the expression of HIF-1alpha in serum of primary hepatocellular carcinoma (PHC) and effects on tumor invasion and metastasis.
The serum HIF-1alpha level from 60 cases of PHC patients and 60 cases of normal healthy individuals were detected by ELISA.
The serum HIF-1alpha level of the PHC group was (161.14+/-80.79) pg/mL, the control group was (22.67+/-8.47) pg/mL, compared the different was significant (P<0.05). The serum HIF-1alpha level of distant metastasis of tumor group was (225.15+/-108.16) pg/mL, the no distant metastasis of tumor group was (101.26+/-55.18) pg/mL, compared the different was significant (P<0.05). The serum HIF-1alpha level of portal vein tumor thrombus group was (255.45+/-122.23) pg/mL. The no portal vein tumor thrombus group was (139.97+/-71.49) pg/mL, compared the different was significant (P<0.05). The serum HIF-1alpha level of tumor capsule group was (99.97+/-55.75) pg/mL. The no tumor capsule group was (187.36+/-91.52) pg/mL, compared the different was significant (P<0.05). The serum HIF-1alpha level was no significantly different among the different age, gender, TNM stage and tumor diameter.
The serum HIF-1alpha has high expression in PHC. The serum HIF-1alpha expression has close relation with the whether distant metastasis of tumor, portal vein tumor thrombus and tumor capsule. The serum HIF-1alpha level can predict the invasion and metastasis of PHC.
研究缺氧诱导因子-1α（HIF-1α）在原发性肝细胞癌（PHC）患者血清中的表达及其对肿瘤侵袭和转移的影响。
采用酶联免疫吸附测定（ELISA）法检测60例PHC患者及60例正常健康个体血清中HIF-1α水平。
PHC组血清HIF-1α水平为（161.14±80.79）pg/mL，对照组为（22.67±8.47）pg/mL，差异有统计学意义（P<0.05）。有远处转移的肿瘤组血清HIF-1α水平为（225.15±108.16）pg/mL，无远处转移的肿瘤组为（101.26±55.18）pg/mL，差异有统计学意义（P<0.05）。门静脉癌栓组血清HIF-1α水平为（255.45±122.23）pg/mL，无门静脉癌栓组为（139.97±71.49）pg/mL，差异有统计学意义（P<0.05）。有肿瘤包膜组血清HIF-1α水平为（99.97±55.75）pg/mL，无肿瘤包膜组为（187.36±91.52）pg/mL，差异有统计学意义（P<0.05）。血清HIF-1α水平在不同年龄、性别、TNM分期及肿瘤直径之间差异无统计学意义。
PHC患者血清HIF-1α呈高表达。血清HIF-1α表达与肿瘤是否发生远处转移、门静脉癌栓及肿瘤包膜密切相关。血清HIF-1α水平可预测PHC的侵袭和转移。
