Zhu Xinle, Wang Shuhuai, Liu Qi, Xu Qian, Zhang Congmin, Xu Shixin, Wang Xia, Li Dan, Hu Haiyan
China Institute of Veterinary Drug Control, Beijing 100081, People's Republic of China.
J AOAC Int. 2011 May-Jun;94(3):839-46.
An HPLC/MS/MS method was developed for the simultaneous determination of the following benzimidazole anthelmintics and metabolites in plasma: flubendazole, albendazole, fenbendazole, mebendazole, thiabendazole, hydrolyzed flubendazole, albendazole sulfoxide, albendazole sulfone, albendazole aminosulfone, oxfendazole, fenbendazole sulfone, aminomebendazole, hydroxymebendazole, and 5-hydroxythiabendazole. The sample preparation process involved a pH-dependent extraction of the analytes. Chromatographic separation was performed on a C18 column with a mobile phase gradient starting with methanol-water (20 + 80, v/v) containing 0.1% formic acid. The overall average recoveries of the analytes based on a matrix-matched calibration ranged from 75.0 to 120.0%, with RSD values of <20.0%. The LODs ranged from 0.08 to 2.0 microg/kg and the LOQs from 0.3 to 5.0 microg/kg. The validated method was used in pharmacokinetic studies of benzimidazole compounds in rabbits, and the elimination of the metabolites was measured quantitatively.
建立了一种HPLC/MS/MS方法，用于同时测定血浆中的以下苯并咪唑驱虫药及其代谢物：氟苯达唑、阿苯达唑、芬苯达唑、甲苯达唑、噻苯达唑、水解氟苯达唑、阿苯达唑亚砜、阿苯达唑砜、阿苯达唑氨基砜、奥芬达唑、芬苯达唑砜、氨基甲苯达唑、羟基甲苯达唑和5-羟基噻苯达唑。样品制备过程包括根据pH值对分析物进行萃取。色谱分离在C18柱上进行，流动相梯度起始于含0.1%甲酸的甲醇-水（20 + 80，v/v）。基于基质匹配校准的分析物总体平均回收率为75.0%至120.0%，相对标准偏差值<20.0%。检测限范围为0.08至2.0微克/千克，定量限范围为0.3至5.0微克/千克。该验证方法用于兔体内苯并咪唑化合物的药代动力学研究，并对代谢物的消除进行了定量测定。
