Deneva-Koycheva Tanya I, Vladimirova-Kitova Lyudmila G, Angelova Evgenia A, Tsvetkova Todorka Z
Department of Clinical Laboratory, Clinic of Cardiology, Medical University, Plovdiv, Bulgaria.
Folia Med (Plovdiv). 2011 Apr-Jun;53(2):22-8. doi: 10.2478/v10153-010-0033-y.
Endothelial dysfunction is increasingly recognized as an important early feature of vascular disease. As the damage to endothelium is a key underlying factor in the development and progression of atherosclerotic processes, markers of endothelial abnormalities have been sought. Increased expression of cell adhesion molecules (CAMs) on the vascular endothelium has been postulated to play a significant role in atherogenesis. Both in vitro and in vivo studies have suggested that different risk factors of atherosclerosis may increase expression of CAMs. The elevated level of soluble forms of CAMs in circulation is associated with a higher risk to future cardiovascular events in subjects predisposed to atherosclerosis
To determine the reference range for serum concentration of soluble cell adhesion molecules - slCAM-1, sVCAM-1, sE-selectin, sP-selectin.
We studied 110 healthy people of Bulgarian nationality aged 18-65. The selection criteria for the reference group were made in accordance with the requirements of the International Federation of Clinical Chemistry (IFCC). Serum concentrations of CAMs were analysed by means of ELISA assay.
The results are presented as central 95% interval and 0.90 confidence interval of the reference range. Reference ranges were determined for sICAM-1 (128.9 - 347.48 ng/ml), sVCAM-1 (170.42 - 478.36 ng/ml), sE-selectin (9.15 - 65.19 ng/ml) and sP-selectin (101.86 - 209.7 ng/ml). As we found no sex-related differences in the CAMs concentrations (p > 0.05) there needed to be no separate reference intervals for men and women. The single-factor dispersion analysis we used in analysing the effect of age found no age-related dependence (p > 0.05, F = 1.038) for the serum CAM concentrations in the 18-65 age range, which means that it is not necessary to establish reference intervals for smaller age ranges in this age group.
The reference ranges for slCAM-1, sVCAM-1, sE-selectin, sP-selectin computed in accordance with the results distribution can be used as baseline criteria in clinical laboratory studies.
内皮功能障碍日益被认为是血管疾病的一个重要早期特征。由于内皮损伤是动脉粥样硬化进程发展和进展的关键潜在因素，人们一直在寻找内皮异常的标志物。血管内皮细胞黏附分子（CAMs）表达增加被认为在动脉粥样硬化形成中起重要作用。体外和体内研究均表明，动脉粥样硬化的不同危险因素可能会增加CAMs的表达。循环中可溶性CAMs水平升高与易患动脉粥样硬化的受试者未来发生心血管事件的风险较高相关。
确定可溶性细胞黏附分子——可溶性细胞间黏附分子-1（slCAM-1）、可溶性血管细胞黏附分子-1（sVCAM-1）、可溶性E-选择素（sE-选择素）、可溶性P-选择素（sP-选择素）血清浓度的参考范围。
我们研究了110名年龄在18至65岁之间的保加利亚籍健康人。参考组的选择标准是根据国际临床化学联合会（IFCC）的要求制定的。通过酶联免疫吸附测定法（ELISA）分析CAMs的血清浓度。
结果以参考范围的中心9
5%区间和0.90置信区间表示。确定了sICAM-1（128.9 - 347.48 ng/ml）、sVCAM-1（170.42 - 478.36 ng/ml）、sE-选择素（9.15 - 65.19 ng/ml）和sP-选择素（101.86 - 209.7 ng/ml）的参考范围。由于我们发现CAMs浓度不存在性别差异（p>0.05），因此无需为男性和女性分别设定参考区间。我们在分析年龄影响时使用的单因素离散分析发现，在18至65岁年龄范围内，血清CAM浓度不存在年龄相关性依赖（p>0.05，F = 1.038），这意味着在该年龄组中无需为更小的年龄范围建立参考区间。
根据结果分布计算出的slCAM-1、sVCAM-1、sE-选择素和sP-选择素的参考范围可作为临床实验室研究的基线标准。
