McDonald J W, Silverstein F S, Johnston M V
Neuroscience and Medical Scientist Training Program, University of Michigan Medical School, Ann Arbor.
Neurosci Lett. 1990 Feb 5;109(1-2):234-8. doi: 10.1016/0304-3940(90)90569-u.
We evaluated the neuroprotective effects of systemically administered magnesium against N-methyl-D-aspartate (NMDA)-mediated brain injury in perinatal rats. Postnatal day (PND) 7 rats received unilateral intrastriatal injections of 25 nmol NMDA followed 15 min later by single or multiple doses of magnesium intraperitoneally (i.p.). Animals were sacrificed five days later and the severity of brain injury was assessed by comparison of the weights of the injected and contralateral cerebral hemispheres. NMDA injection reduced the weight of the injected cerebral hemisphere by 31 +/- 3%. Single doses of magnesium reduced the severity of NMDA-induced brain injury in a dose-dependent fashion (2 mmol/kg, 29 +/- 11% protection; 3 mmol/kg, 52 +/- 12% protection; 4 mmol/kg, 62 +/- 7% protection). Multiple doses of magnesium reduced brain injury by 65 +/- 4%. These data demonstrate that systemically administered magnesium antagonizes the neurotoxic effects of NMDA in vivo in perinatal rats.
我们评估了全身给予镁对围产期大鼠N-甲基-D-天冬氨酸（NMDA）介导的脑损伤的神经保护作用。出生后第7天（PND7）的大鼠单侧纹状体内注射25 nmol NMDA，15分钟后腹腔内（i.p.）单次或多次给予镁。五天后处死动物，通过比较注射侧和对侧大脑半球的重量来评估脑损伤的严重程度。NMDA注射使注射侧大脑半球的重量减少了31±3%。单次给予镁以剂量依赖的方式降低了NMDA诱导的脑损伤的严重程度（2 mmol/kg，保护率为29±11%；3 mmol/kg，保护率为52±12%；4 mmol/kg，保护率为62±7%）。多次给予镁使脑损伤减少了65±4%。这些数据表明，全身给予镁可在体内拮抗围产期大鼠NMDA的神经毒性作用。
