乌司他丁和多西他赛对乳腺癌侵袭及 uPA、uPAR 和 ERK 表达的影响。
Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK.
机构信息
Department of Breast, Pancreas, and Thyroid Surgery Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, China.
出版信息
J Exp Clin Cancer Res. 2011 Jul 29;30(1):71. doi: 10.1186/1756-9966-30-71.
OBJECTIVE
To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells.
METHODS
The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry.
RESULTS
- Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel.
CONCLUSION
Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin.
目的
研究乌司他丁和多西他赛对乳腺癌细胞侵袭以及 uPA、uPAR 和 ERK 表达的影响，选用乳腺癌 MDA-MB-231 和 MCF-7 细胞进行实验。
方法
分别用 PBS、乌司他丁、多西他赛和乌司他丁加多西他赛处理裸鼠，采用 Transwell 法检测细胞侵袭能力，实时 PCR 和 Western blot 检测 uPA、uPAR 和 ERK 的表达，免疫组化法检测裸鼠 uPA、uPAR 和 p-ERK 蛋白水平。
结果
1）乌司他丁、多西他赛和乌司他丁加多西他赛处理均显著抑制 MDA-MB-231 和 MCF-7 细胞侵袭；2）uPA、uPAR 和 ERK1/2 的 mRNA 和蛋白水平被乌司他丁抑制，被多西他赛增强。
结论
乌司他丁能增强多西他赛对乳腺癌细胞侵袭的抑制作用，其作用机制可能与明显抑制 uPA、uPAR 和 p-ERK 表达有关。
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