Ikeda Daisuke, Nihei Yoshiaki, Ono Yosuke, Watabe Shugo
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Japan.
Mar Genomics. 2010 Mar;3(1):1-9. doi: 10.1016/j.margen.2009.12.001. Epub 2010 Jan 12.
Three embryonic myosin heavy chain (MYH) genes >> (MYHs) including MYH(emb1), MYH(emb2) and MYH(emb3) and encoding a C-terminal part of MYH were previously cloned and demonstrated to be expressed transiently in this order during development of common carp Cyprinus carpio embryos. The present study determined the full-length cDNA nucleotide sequences encoding the motor domain of the three MYHs, suggesting the implication of loop 1 and loop 2 sequences for the differences in the motor functions. Phylogenetic analysis based on the full-length amino acid sequences showed that MYH(emb1) and MYH(emb2) both belong to the fast types, though clearly differ from fast-type MYHs expressed in adult fast muscle previously reported. In contrast, MYH(emb3) was in a clade containing slow/cardiac type. Whole-mount immunostaining and in situ hybridization showed that the transcripts of the three embryonic MYHs are localized in the same or different cranial muscles of common carp larvae, suggesting that the three MYHs function cooperatively or individually in various cranial muscles.
三个胚胎肌球蛋白重链（MYH）基因>>（MYHs），包括MYH(emb1)、MYH(emb2)和MYH(emb3)，它们编码MYH的C末端部分，先前已被克隆，并证明在鲤鱼胚胎发育过程中按此顺序瞬时表达。本研究确定了编码这三种MYHs运动结构域的全长cDNA核苷酸序列，表明环1和环2序列与运动功能差异有关。基于全长氨基酸序列的系统发育分析表明，MYH(emb1)和MYH(emb2)均属于快速类型，尽管与先前报道的成年快肌中表达的快速型MYHs明显不同。相比之下，MYH(emb3)属于包含慢肌/心肌类型的一个分支。整装免疫染色和原位杂交表明，三种胚胎MYHs的转录本定位于鲤鱼幼体相同或不同的头部肌肉中，这表明这三种MYHs在各种头部肌肉中协同或单独发挥作用。
