在比目鱼（欧洲黄盖鲽）基因组中鉴定出一个类Tc1转座子整合位点：反向PCR方法的一种新应用。

Identification of a Tc1-like transposon integration site in the genome of the flounder (Platichthys flesus): a novel use of an inverse PCR method.

作者信息

Poćwierz-Kotus Anita, Burzyński Artur, Wenne Roman

机构信息

Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Powstancow Warszawy, Sopot, Poland.

出版信息

Mar Genomics. 2010 Mar;3(1):45-50. doi: 10.1016/j.margen.2010.03.001. Epub 2010 May 5.

DOI:10.1016/j.margen.2010.03.001

PMID:21798196

Abstract

The inverse PCR method has been developed and applied employed for the identification of the integration sites of the Tc1-like transposons in the genome of the flounder, Platichthys flesus. One Tc1-like insertion instance was recognized and characterized, demonstrating an efficiency of the method for determining of transposon integration sites. The similarity of the sequence flanking transposon (SFT) to reverse transcriptase sequences (RVT) was demonstrated. It is likely that the insertion took place within currently degenerated LINE (long interspersed nuclear elements) retrotransposon.

摘要

已开发出反向PCR方法并将其应用于鉴定比目鱼（欧洲黄盖鲽）基因组中类Tc1转座子的整合位点。识别并表征了一个类Tc1插入实例，证明了该方法在确定转座子整合位点方面的有效性。证明了转座子侧翼序列（SFT）与逆转录酶序列（RVT）的相似性。插入很可能发生在当前已退化的LINE（长散在核元件）逆转座子内。