Vilniaus Gedimino technikos universitetas, Vilnius, Lithuania.
Biochem Biophys Res Commun. 2011 Sep 9;412(4):537-42. doi: 10.1016/j.bbrc.2011.07.042. Epub 2011 Jul 26.
The purpose of this research was to analyse experimental data concerning cytosolic calcium concentration in view of the mechanisms involved in calcium fluxes in human platelets. The parameters of model curves are related to the properties of the entities responsible for control or maintenance of cytosolic calcium concentration. It has been shown that: (a) biphasicity of increase in cytosolic calcium concentration caused by inhibition of SERCAs either by TBHQ and TG or by TG alone is related to fast and slow discharge of acidic calcium stores and DTS; (b) biphasicity of decline in cytosolic calcium concentration after its rise caused by stimulation of platelets by the agonists is related to non-synchronous extrusion of calcium by PMCA and NCX; (c) NCX is active only in calcium containing medium: calcium ion(s) are necessary to be bound to the site(s) located on the medium-facing side of the (macro)molecule; (d) PMCA is likely to be activated either by binding calcium ion(s) to the site(s) located on its cytosol-facing side or by unbinding identical ion(s) from the site(s) on its medium-facing side.
本研究旨在分析与人类血小板钙流相关的胞浆钙浓度变化机制的实验数据。模型曲线的参数与负责控制或维持胞浆钙浓度的实体的特性有关。结果表明：(a) 由 TBHQ 和 TG 或 TG 单独抑制 SERCAs 引起的胞浆钙浓度增加的双相性与酸性钙库和 DTS 的快速和缓慢释放有关；(b) 由激动剂刺激血小板引起的胞浆钙浓度升高后的下降的双相性与 PMCA 和 NCX 非同步的钙外排有关；(c) NCX 仅在含钙介质中活跃：钙离子必须与位于（大分子）分子面向介质侧的位点结合；(d) PMCA 可能通过将钙离子结合到其胞质侧的位点或从其面向介质侧的位点上解结合相同的离子而被激活。
