Department of General Surgery, The Second Affiliated Hospital of Nan Chang University, 330006 Nanchang City, Jiangxi Province, China.
Cytokine. 2011 Nov;56(2):167-73. doi: 10.1016/j.cyto.2011.06.018. Epub 2011 Jul 27.
Associations between five polymorphisms of vascular endothelial growth factor (i.e., VEGF +936C/T, -1154A/G, -2578C/A, -634G/C and -460T/C) and risk of breast cancer have been extensively studied, and the currently available results are inconclusive. Therefore, we performed this meta-analysis to further study the associations. The databases of Pubmed, Embase and CNKI were retrieved up to April 1st, 2010. The pooled ORs and 95% CIs were used to assess the strength of the associations. A total of 10 case-control studies with 8175 cases and 8528 controls were included in this study. The overall results of combined analyses showed that five polymorphisms of VEGF were not associated with risk of breast cancer [ORs (95% CIs): 1.03 (0.84-1.27) for CC vs. TT for +936C/T, 0.95 (0.81-1.12) for AA vs. GG for -1154A/G, 1.01 (0.90-1.14) for CC vs. AA for -2578C/A, 1.02 (0.90-1.16) for GG vs. CC for -634G/C and 0.86 (0.68-1.09) for TT vs. CC for -460T/C]. When subgroup analyses by ethnicity for VEGF +936C/T and -634G/C, the results suggested that +936C/T was not associated with the risk of breast cancer for either Asians [1.40 (0.92-2.13) for CC vs. TT and CC+CT vs. TT: 1.38 (0.91-2.10) for CC+CT vs. TT] or Caucasians [0.93 (0.73-1.19) for CC vs. TT and 0.91 (0.72-1.16) for CC+CT vs. TT], and -634G/C was not associated with the breast cancer for Caucasians [1.07 (0.92-1.24) for GG vs. CC and 1.05 (0.91-1.21) for GG+GC vs. CC]. In addition, when excluding one study, which was out of Hardy-Weinberg equilibrium for VEGF +963C/T and whose controls were from both patients and healthy people, the negative results were also persistent, and ORs (95% CIs) were 1.04 (0.84-1.29) for CC vs. TT, 1.03 (0.83-1.27) for (CC+CT) vs. TT. This meta-analysis suggests that the VEGF +936C/T, -1154A/G, -2578C/A, -634G/C and -460T/C may be not associated with risk of breast cancer development based on the currently available studies, especially for Caucasians. More well designed studies with larger sample size on different ethnicities are needed to further assess the associations.
血管内皮生长因子（VEGF）的五个多态性（即 VEGF +936C/T、-1154A/G、-2578C/A、-634G/C 和 -460T/C）与乳腺癌风险之间的关联已被广泛研究，但目前的结果尚无定论。因此，我们进行了这项荟萃分析，以进一步研究这些关联。检索了 Pubmed、Embase 和 CNKI 数据库，截至 2010 年 4 月 1 日。使用合并的 OR 和 95%CI 来评估关联的强度。这项研究共纳入了 10 项病例对照研究，包括 8175 例病例和 8528 例对照。综合分析的总体结果表明，VEGF 的五个多态性与乳腺癌风险无关[ORs（95%CI）：+936C/T 的 CC 对 TT（1.03，0.84-1.27），-1154A/G 的 AA 对 GG（0.95，0.81-1.12），-2578C/A 的 CC 对 AA（1.01，0.90-1.14），-634G/C 的 GG 对 CC（1.02，0.90-1.16）和-460T/C 的 TT 对 CC（0.86，0.68-1.09）]。对 VEGF +936C/T 和 -634G/C 进行亚组分析时，结果表明，亚洲人 [+936C/T 的 CC 对 TT（1.40，0.92-2.13）和 CC+CT 对 TT（1.38，0.91-2.10）]或白种人 [+936C/T 的 CC 对 TT（0.93，0.73-1.19）和 CC+CT 对 TT（0.91，0.72-1.16）]的 +936C/T 与乳腺癌风险无关，-634G/C 与白种人 [-634G/C 的 GG 对 CC（1.07，0.92-1.24）和 GG+GC 对 CC（1.05，0.91-1.21）]的乳腺癌风险无关。此外，当排除一项 VEGF +963C/T 不符合 Hardy-Weinberg 平衡且其对照来自患者和健康人群的研究时，阴性结果仍然持续存在，ORs（95%CI）为 CC 对 TT（1.04，0.84-1.29），（CC+CT）对 TT（1.03，0.83-1.27）。本荟萃分析表明，目前的研究结果提示 VEGF +936C/T、-1154A/G、-2578C/A、-634G/C 和 -460T/C 与乳腺癌发病风险可能无关，特别是对于白种人。需要在不同种族中进行更大样本量的设计更好的研究来进一步评估这些关联。
