Department of Medical Oncology, Medical School of Nanjing University, Jinling Hospital, 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
World J Gastroenterol. 2011 Jul 7;17(25):3002-11. doi: 10.3748/wjg.v17.i25.3002.
To investigate whether cisplatin (DDP) enhances the anti-tumor activity of cytokine- induced killer (CIK) cells in a murine colon adenocarcinoma model.
Tumor size and weight served as indicators of therapeutic response. Immunohistochemistry was performed to observe intratumoral lymphocyte infiltration and tumor microvessel density. Changes in the percentage of regulatory T (Treg) cells within the spleens of tumor-bearing mice preconditioned with DDP were monitored using flow cytometry.
A marked T cell-dependent, synergistic anti-tumor effect of the combined therapy was observed (1968 ± 491 mm³ vs 3872 ± 216 mm³; P = 0.003). Preconditioning chemotherapy with DDP augmented the infiltration of CD3+ T lymphocytes into the tumor mass and reduced the percentage of both intratumoral and splenic Treg cells.
Preconditioning with DDP markedly enhances the efficacy of adoptively transferred CIK cells, providing a potential clinical modality for the treatment of patients with colorectal cancer.
研究顺铂（DDP）是否增强细胞因子诱导的杀伤（CIK）细胞在小鼠结肠腺癌模型中的抗肿瘤活性。
肿瘤大小和重量作为治疗反应的指标。免疫组织化学用于观察肿瘤内淋巴细胞浸润和肿瘤微血管密度。用流式细胞术监测荷瘤小鼠预先用 DDP 处理后脾脏中调节性 T（Treg）细胞的百分比变化。
观察到联合治疗具有显著的 T 细胞依赖性协同抗肿瘤作用（1968 ± 491 mm³ 对 3872 ± 216 mm³；P = 0.003）。DDP 预处理化疗增强了 CD3+T 淋巴细胞向肿瘤组织的浸润，并降低了肿瘤内和脾脏 Treg 细胞的百分比。
DDP 预处理可显著增强过继转移的 CIK 细胞的疗效，为治疗结直肠癌患者提供了一种潜在的临床方法。
