CUDaM, University of Milano Bicocca, Milano, Italy.
Anal Bioanal Chem. 2011 Oct;401(6):2019-23. doi: 10.1007/s00216-011-5252-4. Epub 2011 Jul 29.
Bones are potentially the best age indicators in a stratigraphic study, because they are closely related to the layer in which they are found. Collagen is the most suitable fraction and is the material normally used in radiocarbon dating. Bone contaminants can strongly alter the carbon isotopic fraction values of the samples, so chemical pretreatment for (14)C dating by accelerator mass spectrometry (AMS) is essential. The most widespread method for collagen extraction is based on the Longin procedure, which consists in HCl demineralization to dissolve the inorganic phase of the samples, followed by dissolution of collagen in a weak acid solution. In this work the possible side effects of this procedure on a modern bone are presented; the extracted collagen was analyzed by ATR-IR spectroscopy. An alternative procedure, based on use of HF instead of HCl, to minimize unwanted degradation of the organic fraction, is also given. A study by ATR-IR spectroscopic analysis of collagen collected after different demineralization times and with different acid volumes, and a study of an archaeological sample, are also presented.
骨骼是地层研究中最理想的年龄指标，因为它们与所处的地层密切相关。胶原蛋白是最合适的部分，也是通常用于放射性碳测年的材料。骨骼中的污染物会强烈改变样品的碳同位素分数值，因此，对加速器质谱（AMS）进行 (14)C 测年的化学前处理是必不可少的。最广泛使用的胶原蛋白提取方法基于 Longin 程序，该程序包括 HCl 脱矿质，以溶解样品的无机相，然后在弱酸溶液中溶解胶原蛋白。本工作介绍了该程序对现代骨骼可能产生的副作用；提取的胶原蛋白通过衰减全反射-红外光谱（ATR-IR）进行分析。还给出了一种替代程序，使用 HF 代替 HCl，以尽量减少有机部分的不必要降解。还介绍了通过 ATR-IR 光谱分析收集的胶原蛋白在不同脱矿时间和不同酸体积后的情况，以及对一个考古样本的研究。
