Li Qian, Cui Zhisong, Zhao Aifen, Gao Wei, Zheng Li
College of Life Science, Ludong University, Yantai 264025, China.
Wei Sheng Wu Xue Bao. 2011 May;51(5):648-55.
To identify and characterize a hydrocarbon-degrading bacterium isolated from the sediment of the Yellow Sea.
We used 16S rRNA gene sequences based phylogenetic analysis, physiological and biochemical characterization, DNA G + C content assaying, determination of cellular fatty acids, testing of carbon sources and respiratory lipoquinone and experiment of DNA-DNA relatedness. Its capability of degrading aliphatic hydrocarbons in ONR7a media supplemented with nine n-alkanes, separately, as sole source of carbon and energy was further determined.
The Gram-negative isolate PY97S was a member of the genus Marinobacter, catalase-and oxidase-positive, and with Q-9 as its predominant respiratory lipoquinone. The similarity between its 16S rRNA gene and that of its most closely related type strain in GenBank Marinobacter koreensis DD-M3(T) was 96.93%, and their level of DNA relatedness was 46.7%. The appropriate temperature for its growth ranged from 15 degrees C to 35 degrees C with the optimum of 30 degrees C, the appropriate initial acidity from pH 6.0 to 9.5 with the optimum of pH 7.0, and the appropriate salinity (NaCl) from 0% to 10% with the optimum of 0%. It metabolized many carbohydrates and organic acids and was sensitive to diverse antibiotics including ampicillin and piperacillin. The G + C content of its genomic DNA was 48.2 mol%. The major fatty acids were 2-methyl C15:0 (29.97%), C16: 1omega7c (27.22%), C12:0 (22.22%) and C16: 1omega9c (5.73%).
The isolate PY97S was identified as a petroleum hydrocarbon-degrading novel species of genus Marinobacter, holding the potential of being applied in the bioremediation of oil spill.
鉴定并表征从黄海沉积物中分离出的一种烃降解细菌。
我们采用基于16S rRNA基因序列的系统发育分析、生理生化特性分析、DNA G + C含量测定、细胞脂肪酸测定、碳源和呼吸性脂醌测试以及DNA - DNA相关性实验。进一步测定了其在分别添加9种正构烷烃作为唯一碳源和能源的ONR7a培养基中降解脂肪烃的能力。
革兰氏阴性分离株PY97S属于海杆菌属，过氧化氢酶和氧化酶呈阳性，以Q - 9作为主要呼吸性脂醌。其16S rRNA基因与GenBank中最相近的模式菌株海杆菌属韩国海杆菌DD - M3(T)的相似性为96.93%，它们的DNA相关性水平为46.7%。其生长的适宜温度为15℃至35℃，最适温度为30℃；适宜的初始酸度为pH 6.0至9.5，最适pH为7.0；适宜的盐度（NaCl）为0%至10%，最适盐度为0%。它能代谢多种碳水化合物和有机酸，对包括氨苄西林和哌拉西林在内的多种抗生素敏感。其基因组DNA的G + C含量为48.2 mol%。主要脂肪酸为2 - 甲基C15:0（29.97%）、C16: 1ω7c（27.22%）、C12:0（22.22%）和C16: 1ω9c（5.73%）。
分离株PY97S被鉴定为海杆菌属的一种石油烃降解新物种，具有应用于溢油生物修复的潜力。
