Department of Zoology, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand.
Exp Parasitol. 2011 Oct;129(2):101-6. doi: 10.1016/j.exppara.2011.07.009. Epub 2011 Jul 23.
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus. The medium was optimized by sequentially testing the suitability of different levels of osmolality, different commercially available media, and different concentrations of supplemented chicken serum, while controlling for genetic variation among cultures. Philophthalmus sp. rediae survived up to 56 days in cultures of the best tested medium, remaining active and continuously shedding cercariae. The broader suitability of the culture medium was tested using five other trematode species from different families (using either the same or other marine snails as first intermediate hosts): Galactosomum sp., Acanthoparyphium sp., Maritrema novaezealandensis, Curtuteria australis, and an undescribed species of the family Opecoelidae. Survivorship of rediae and sporocysts from these species ranged from eight days to 42 days. The culture procedures developed here can therefore be used in the future as a system under which to culture marine trematode parthenitae for experimental studies.
在宿主外培养寄生虫（即体外培养）对于寄生虫学研究的几个方面至关重要。在这里，我们使用中间宿主锥实螺（Zeacumantus subcarinatus）中的 Philophthalmus sp. 尾蚴来优化海洋吸虫的原尾蚴培养物。通过依次测试不同渗透压水平、不同市售培养基以及不同浓度补充鸡血清的适用性，同时控制培养物之间的遗传变异，来优化培养基。Philophthalmus sp. 尾蚴在最佳测试培养基的培养物中存活了长达 56 天，保持活跃并持续释放尾蚴。使用来自不同科的另外五种吸虫（使用相同或其他海洋蜗牛作为第一中间宿主）测试了培养基的更广泛适用性：Galactosomum sp.、Acanthoparyphium sp.、Maritrema novaezealandensis、Curtuteria australis 和一种未描述的 Opecoelidae 科物种。这些尾蚴和胞囊的存活率从 8 天到 42 天不等。因此，这里开发的培养程序将来可以用作培养海洋吸虫原尾蚴进行实验研究的系统。
