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由烃类降解和重金属耐受菌弗格森埃希氏菌 KLU01 产生的新型脂肽生物表面活性剂作为生物修复的潜在工具。

Novel lipopeptide biosurfactant produced by hydrocarbon degrading and heavy metal tolerant bacterium Escherichia fergusonii KLU01 as a potential tool for bioremediation.

机构信息

Division of Molecular and Cellular Biology, Department of Biotechnology, Kalasalingam University, Anand Nagar, Krishnankoil 626126, Tamilnadu, India.

出版信息

Bioresour Technol. 2011 Oct;102(19):9291-5. doi: 10.1016/j.biortech.2011.06.094. Epub 2011 Jul 2.

DOI:10.1016/j.biortech.2011.06.094
PMID:21802283
Abstract

Escherichia fergusonii KLU01, a propitious bacterial strain isolated from oil contaminated soil was identified to be hydrocarbon degrading, heavy metal tolerant and a potent producer of biosurfactant using diesel oil as the sole carbon and energy source. The biosurfactant produced by the strain was characterized to be a lipopeptide. The minimum active dose and critical micelle concentration of the biosurfactant were found as 0.165±0.08 μg and 36 mg/L, respectively. In spite of being an excellent emulsifier, the biosurfactant showed an incredible stability at extremes of temperature, pH and at various concentrations of NaCl, CaCl₂ and MgCl₂. Also the bacterium manifested tolerance towards Manganese, Iron, Lead, Nickel, Copper and Zinc. The strain emerges as a new class of biosurfactant producer with potential environmental and industrial applications, especially in hydrocarbon degradation and heavy metal bioremediation.

摘要

弗格森埃希氏菌 KLU01 是从污染土壤中分离出来的一种有利细菌菌株,被鉴定为能够降解碳氢化合物、耐受重金属,并且能够以柴油为唯一碳源和能源大量生产生物表面活性剂。该菌株产生的生物表面活性剂被鉴定为脂肽。生物表面活性剂的最低有效剂量和临界胶束浓度分别为 0.165±0.08μg 和 36mg/L。尽管是一种优秀的乳化剂,但该生物表面活性剂在极端温度、pH 值以及不同浓度的 NaCl、CaCl₂ 和 MgCl₂ 下表现出令人难以置信的稳定性。此外,该细菌对锰、铁、铅、镍、铜和锌也表现出耐受性。该菌株是一种新型的生物表面活性剂产生菌,具有潜在的环境和工业应用价值,特别是在碳氢化合物降解和重金属生物修复方面。

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