Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, 20131 Milano, Italy.
Anal Biochem. 2011 Nov 1;418(1):164-6. doi: 10.1016/j.ab.2011.07.004. Epub 2011 Jul 14.
Here we combine the use of fluorescence-enhancing silicon substrates coated by copoly(DMA-NAS-MAPS), a ter-copolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS), with an efficient dynamic incubation to overcome mass transport limitations and obtain femtomolar limits of detection. The high sensitivity was obtained with a conventional microarray scanner without the use of any sophisticated detection strategy or protocol. When the method was applied, an improvement of the analytical sensitivity of approximately three orders of magnitude was achieved for antibody detection when compared with the same assay performed on regular glass slides and static conditions. Moreover, limits of detection of 45 and 54 pg/ml were obtained for hepatitis B superficial antigen and HIV p24 antigen, respectively.
在这里，我们将荧光增强硅基底与共聚物（DMA-NAS-MAPS）结合使用，该共聚物基于 N,N-二甲基丙烯酰胺（DMA）、N-丙烯酰氧基琥珀酰亚胺（NAS）和 3-（三甲氧基硅基）丙基-甲基丙烯酸酯（MAPS），并采用高效的动态孵育来克服质量传输限制，从而获得飞摩尔级别的检测限。该方法使用常规的微阵列扫描仪即可实现高灵敏度，无需使用任何复杂的检测策略或方案。当该方法应用于抗体检测时，与在常规玻片上进行的相同检测相比，其分析灵敏度提高了约三个数量级。此外，乙型肝炎表面抗原和 HIV p24 抗原的检测限分别为 45pg/ml 和 54pg/ml。
