Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Food Chem Toxicol. 2011 Oct;49(10):2631-7. doi: 10.1016/j.fct.2011.07.008. Epub 2011 Jul 23.
Cis-diamminedichloroplatinum II (CDDP)-induced nephrotoxicity is associated with the overproduction of reactive oxygen species. tert-Butylhydroquinone (tBHQ) is a compound widely used as food antioxidant. The purpose of this study was to investigate the ability of tBHQ to prevent the nephrotoxic effect of CDDP in rats as well as the mechanisms involved. Thirty-six Wistar rats divided in the following groups were used: control, tBHQ (12.5mg/kg), CDDP (7.5mg/kg) and tBHQ+CDDP. Twenty-four h urine was collected at the beginning and at the end of the experiment and the rats were sacrificed 72h after CDDP-administration. Histological studies were performed and markers of renal function and oxidative/nitrosative stress were measured. In addition, the activity of the following antioxidant enzymes was measured: glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST). CDDP-induced renal dysfunction, structural damage and oxidative/nitrosative were prevented by tBHQ. In addition, tBHQ completely prevented the CDDP-induced fall in GPx and GST activities. In conclusion, the present study indicates that the antioxidant activity of tBHQ is associated with its nephroprotective effect against CDDP-induced acute kidney injury in rats.
顺铂（CDDP）诱导的肾毒性与活性氧的过度产生有关。叔丁基对苯二酚（tBHQ）是一种广泛用作食品抗氧化剂的化合物。本研究旨在探讨 tBHQ 预防 CDDP 对大鼠肾毒性的能力以及涉及的机制。使用 36 只 Wistar 大鼠分为以下几组：对照组、tBHQ（12.5mg/kg）、CDDP（7.5mg/kg）和 tBHQ+CDDP。在实验开始和结束时收集 24 小时尿液，并在给予 CDDP 后 72 小时处死大鼠。进行组织学研究，并测量肾功能和氧化/硝化应激的标志物。此外，还测量了以下抗氧化酶的活性：谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）和谷胱甘肽-S-转移酶（GST）。tBHQ 可预防 CDDP 诱导的肾功能障碍、结构损伤和氧化/硝化。此外，tBHQ 完全阻止了 CDDP 诱导的 GPx 和 GST 活性下降。总之，本研究表明，tBHQ 的抗氧化活性与其预防 CDDP 诱导的大鼠急性肾损伤的肾保护作用有关。
