嘌呤支架荧光探针的合成及其在流式细胞术和荧光显微镜中的应用
Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy.
机构信息
Program in Molecular Pharmacology and Chemistry, Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
出版信息
Bioorg Med Chem Lett. 2011 Sep 15;21(18):5347-52. doi: 10.1016/j.bmcl.2011.07.026. Epub 2011 Jul 14.
Abstract
Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
摘要
荧光配体为热休克蛋白 90（Hsp90）的合成含有荧光素异硫氰酸酯（FITC），4-硝基苯并[1,2,5]恶二唑（NBD）或红色位移染料 sulforhodamine 101（Texas Red）缀合到 PU-H71。这两种化合物，PU-H71-FITC2（9）和 PU-H71-NBD1（8），被证明适用于荧光激活的流式细胞术和荧光显微镜检查。因此，这些分子可作为研究异质活细胞群体中 Hsp90 的有用探针。
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