绝缘子 cHS4 对正反馈系统中质粒 DNA 中转基因表达的影响。

Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system.

机构信息

Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.

出版信息

J Biosci Bioeng. 2011 Nov;112(5):432-4. doi: 10.1016/j.jbiosc.2011.07.001. Epub 2011 Jul 29.

DOI:10.1016/j.jbiosc.2011.07.001

Abstract

The effects of a genetic insulator (chicken cHS4) on transgene expression in a positive feedback system using the GAL4-VP16 activator and luciferase reporter plasmids were examined. The introduction of cHS4 enhanced luciferase expression in mouse liver, indicating that this system would be useful for efficient transgene expression.

摘要

研究了利用 GAL4-VP16 激活子和荧光素酶报告质粒的正反馈系统中，一个遗传绝缘子（鸡 cHS4）对转基因表达的影响。cHS4 的引入增强了小鼠肝脏中的荧光素酶表达，表明该系统将有助于高效的转基因表达。