下颌骨血肿细胞作为骨折中骨祖细胞的潜在储存库。

Mandibular hematoma cells as a potential reservoir for osteoprogenitor cells in fractures.

作者信息

Hasegawa Takumi, Miwa Masahiko, Sakai Yoshitada, Niikura Takahiro, Lee Sang Yang, Oe Keisuke, Iwakura Takashi, Kurosaka Masahiro, Komori Takahide

机构信息

Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.

出版信息

J Oral Maxillofac Surg. 2012 Mar;70(3):599-607. doi: 10.1016/j.joms.2011.03.043. Epub 2011 Jul 30.

DOI:10.1016/j.joms.2011.03.043

PMID:21803469

Abstract

PURPOSE

We hypothesized that cells within the mandibular fracture hematoma played an important role in mandibular fracture healing. The objective of this study was to analyze cells in human mandibular fracture hematoma.

PATIENTS AND METHODS

We isolated and analyzed human mandibular fracture hematoma cells (MHCs) and investigated whether MHCs had multilineage mesenchymal differentiation capacity in vitro, similar to bone marrow stromal cells (BMSCs).

RESULTS

Cell-surface markers showed that the adherent MHCs expressed mesenchymal stem cell-related markers, namely CD29, CD44, CD105, and CD166, while lacking hematopoietic markers CD14, CD34, CD45, and CD133. The proliferative potential, osteogenic potential, and adipogenic potential of MHCs were comparable to those of BMSCs. In contrast, the chondrogenic potential of MHCs was inferior to that of BMSCs.

CONCLUSIONS

The role of the mandibular fracture hematoma could be as a presumptive local reservoir for osteogenic progenitors and thus contribute to intramembranous bone healing. Our findings may provide new insights into the mechanism of intramembranous bone healing in membranous bone fractures.

摘要