Hasegawa Takumi, Miwa Masahiko, Sakai Yoshitada, Niikura Takahiro, Lee Sang Yang, Oe Keisuke, Iwakura Takashi, Kurosaka Masahiro, Komori Takahide
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
J Oral Maxillofac Surg. 2012 Mar;70(3):599-607. doi: 10.1016/j.joms.2011.03.043. Epub 2011 Jul 30.
We hypothesized that cells within the mandibular fracture hematoma played an important role in mandibular fracture healing. The objective of this study was to analyze cells in human mandibular fracture hematoma.
We isolated and analyzed human mandibular fracture hematoma cells (MHCs) and investigated whether MHCs had multilineage mesenchymal differentiation capacity in vitro, similar to bone marrow stromal cells (BMSCs).
Cell-surface markers showed that the adherent MHCs expressed mesenchymal stem cell-related markers, namely CD29, CD44, CD105, and CD166, while lacking hematopoietic markers CD14, CD34, CD45, and CD133. The proliferative potential, osteogenic potential, and adipogenic potential of MHCs were comparable to those of BMSCs. In contrast, the chondrogenic potential of MHCs was inferior to that of BMSCs.
The role of the mandibular fracture hematoma could be as a presumptive local reservoir for osteogenic progenitors and thus contribute to intramembranous bone healing. Our findings may provide new insights into the mechanism of intramembranous bone healing in membranous bone fractures.
我们推测下颌骨骨折血肿内的细胞在下颌骨骨折愈合中起重要作用。本研究的目的是分析人类下颌骨骨折血肿中的细胞。
我们分离并分析了人类下颌骨骨折血肿细胞（MHCs），并研究MHCs在体外是否具有与骨髓基质细胞（BMSCs）相似的多谱系间充质分化能力。
细胞表面标志物显示，贴壁的MHCs表达间充质干细胞相关标志物，即CD29、CD44、CD105和CD166，而缺乏造血标志物CD14、CD34、CD45和CD133。MHCs的增殖潜能、成骨潜能和脂肪生成潜能与BMSCs相当。相比之下，MHCs的软骨生成潜能低于BMSCs。
下颌骨骨折血肿的作用可能是作为成骨祖细胞的假定局部储存库，从而促进膜内骨愈合。我们的研究结果可能为膜性骨骨折的膜内骨愈合机制提供新的见解。
