利用含有志贺邻单胞菌血红素转运基因和改良人血红蛋白基因的质粒转化大肠杆菌 BL21(DE3)进行生物反应器培养生产血红蛋白的方法的开发。
Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.
机构信息
Department of Biology, University of Texas of the Permian Basin, Odessa, TX 79762-0001, USA.
出版信息
Appl Environ Microbiol. 2011 Sep;77(18):6703-5. doi: 10.1128/AEM.05712-11. Epub 2011 Jul 29.
Abstract
We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
摘要
我们描述了一种通过在生物反应器中生长的大肠杆菌生产重组人血红蛋白的方法。含有血红蛋白基因和类志贺邻单胞菌血红素转运基因的质粒转化的大肠杆菌 BL21(DE3)达到了 83.64g/L 的细胞干重，并在澄清的裂解物中产生了 11.92g/L 的血红蛋白。
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本文引用的文献
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Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system.在含有志贺邻单胞菌血红素转运系统的大肠杆菌BL21(DE3)中提高重组血红蛋白的产量。
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2
Enhancing stability and expression of recombinant human hemoglobin in E. coli: Progress in the development of a recombinant HBOC source.增强重组人血红蛋白在大肠杆菌中的稳定性和表达：重组血红蛋白氧载体来源开发的进展
Biochim Biophys Acta. 2008 Oct;1784(10):1471-9. doi: 10.1016/j.bbapap.2008.04.012. Epub 2008 May 1.
3
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Trends Biotechnol. 2006 Aug;24(8):372-7. doi: 10.1016/j.tibtech.2006.06.005. Epub 2006 Jul 11.
4
Oxygen carriers: a selected review.氧载体：一篇精选综述。
Transfus Apher Sci. 2006 Feb;34(1):25-32. doi: 10.1016/j.transci.2005.09.005. Epub 2005 Dec 22.
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6
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