[维吾尔药神香草对哮喘大鼠肺组织中T-bet、GATA-3和STAT-3 mRNA表达的影响]

[The effect of Uygur medicine Hyssopus officinalis L on expression of T-bet, GATA-3 and STAT-3 mRNA in lung tissue of asthma rats].

作者信息

Wang Hong-ying, Ding Jian-bing, Halmurat Upur, Hou Min, Xue Zhi-qin, Zhu Ming, Tian Shu-ge, Ma Xiu-min

机构信息

Xinjiang VIP Lab of Great Physician and Pharmacology of Traditional Chinese Medical Formulae, Urumqi, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Aug;27(8):876-9.

PMID:21806885

Abstract

AIM

To study the Uygur medicine Hyssopus officinalis L on T-bet, GATA-3, STAT-3 mRNA levels of asthma rats in order to explore the mechanism of its treatment of asthma.

METHODS

Rats were randomly divided into normal control group, asthma model group and dexamethasone group and water extract of Hyssopus officinalis L high and low dose group. The rats were sensitized with OVA, Al(OH)(3); and DPT vaccine and then challenged with inhalation of aerosolized OVA solution for Preparation of asthma model and the level of T-bet, GATA-3, STAT-3 mRNA were detected with RT-PCR.

RESULTS

The normal control group and model group, model group and treatment group, the expression of T-bet, GATA-3 and STAT-3 mRNA in the lung tissue was statistically significant differences(P<0.05). Compared with model group, after treatment of Hyssopus officinalis L the expression of GATA-3 and STAT-3 mRNA of asthma rats significantly reduced (P<0.05), but the expression of T-bet mRNA was significantly higher (P<0.05).The expression of GATA-3 and STAT-3 mRNA of Hyssopus officinalis L high-dose treatment group was lower than the low-dose treatment group (P<0.05), but T-bet mRNA that was higher(P<0.05). The expression of T-bet mRNA has negative correlation with GATA-3 mRNA (r=-0.696), the expression of STAT-3 mRNA has correlation with T-bet mRNA and GATA-3 mRNA(r=-0.767, 0.772), P<0.05.

CONCLUSION

Hyssopus officinalis L probably regulates the differentiation of Th1, Th2 and Th17 on transcription level to play the role of anti-inflammatory.

摘要

目的

研究维吾尔药神香草对哮喘大鼠T-bet、GATA-3、STAT-3 mRNA水平的影响，以探讨其治疗哮喘的作用机制。

方法

将大鼠随机分为正常对照组、哮喘模型组、地塞米松组及神香草水提取物高、低剂量组。采用卵白蛋白（OVA）、氢氧化铝[Al(OH)₃]及百日咳疫苗致敏大鼠，然后雾化吸入OVA溶液激发，制备哮喘模型，采用逆转录聚合酶链反应（RT-PCR）检测肺组织中T-bet、GATA-3、STAT-3 mRNA水平。

结果

正常对照组与模型组、模型组与各治疗组肺组织中T-bet、GATA-3、STAT-3 mRNA表达差异有统计学意义（P<0.05）。与模型组比较，神香草治疗后哮喘大鼠GATA-3、STAT-3 mRNA表达显著降低（P<0.05），但T-bet mRNA表达显著升高（P<0.05）。神香草高剂量治疗组GATA-3、STAT-3 mRNA表达低于低剂量治疗组（P<0.05），而T-bet mRNA表达高于低剂量治疗组（P<0.05）。T-bet mRNA表达与GATA-3 mRNA表达呈负相关（r=-0.696），STAT-3 mRNA表达与T-bet mRNA、GATA-3 mRNA均有相关性（r=-0.767、0.772），P<0.05。