Ning Han-bing, Wang Yan-hong, Zhang Lian-feng, Li Ji-chang
Department of Digestive Deseases, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Aug;27(8):899-900.
To investigate the effects of gastric dendritic cells(DCs) in H.pylori-associated gastritis.
Mononuclear cells were isolated from gastric samples of endoscoped healthy subjects or patients with gastritis by Ficoll-hypaquetechnique. HLA-DR(+);DCs were further isolated by MACS. Rapid urease test, warthin-starry dyer and C(14);-urea breath test were used to detect H.pylori. The relationship between the quantity of gastric DCs and H.pylori density or inflammatory background were analysed.
The gastric mononuclear cells from H.pylori-infected normal gastric mucosa contained more HLA-DR(+);DCs (17.93 % ) than that from noninfected subjects (4.93%).The quantity of gastric DCs is positively correlated with H.pylori density. The quantity of gastric DCs were significantly higher in moderate and high inflammatory gastric mucosa than that in low inflammatory gastric mucosa. There was no difference in the quantity of gastric DCs between moderate and high inflammatory gastric mucosa.
Successfully isolated gastric DCs. DCs may play a role in the early stage of H.pylori-associated gastritis.
研究胃树突状细胞（DCs）在幽门螺杆菌相关性胃炎中的作用。
采用Ficoll- Hypaque技术从内镜检查的健康受试者或胃炎患者的胃样本中分离单核细胞。通过磁珠分选法进一步分离HLA-DR(+) DCs。采用快速尿素酶试验、沃辛-斯塔里染色法和碳-14尿素呼气试验检测幽门螺杆菌。分析胃DCs数量与幽门螺杆菌密度或炎症背景之间的关系。
幽门螺杆菌感染的正常胃黏膜中的胃单核细胞所含HLA-DR(+) DCs（17.93%）比未感染受试者（4.93%）更多。胃DCs数量与幽门螺杆菌密度呈正相关。中度和高度炎症性胃黏膜中的胃DCs数量显著高于低度炎症性胃黏膜。中度和高度炎症性胃黏膜之间的胃DCs数量无差异。
成功分离出胃DCs。DCs可能在幽门螺杆菌相关性胃炎的早期阶段发挥作用。
